論文 - 詳細
| RRC ID | 55033 |
|---|---|
| 著者 | Osawa T, Kotani T, Kawaoka T, Hirata E, Suzuki K, Nakatogawa H, Ohsumi Y, Noda NN. |
| タイトル | Atg2 mediates direct lipid transfer between membranes for autophagosome formation. |
| ジャーナル | Nat Struct Mol Biol |
| Abstract |
A key event in autophagy is autophagosome formation, whereby the newly synthesized isolation membrane (IM) expands to form a complete autophagosome using endomembrane-derived lipids. Atg2 physically links the edge of the expanding IM with the endoplasmic reticulum (ER), a role that is essential for autophagosome formation. However, the molecular function of Atg2 during ER-IM contact remains unclear, as does the mechanism of lipid delivery to the IM. Here we show that the conserved amino-terminal region of Schizosaccharomyces pombe Atg2 includes a lipid-transfer-protein-like hydrophobic cavity that accommodates phospholipid acyl chains. Atg2 bridges highly curved liposomes, thereby facilitating efficient phospholipid transfer in vitro, a function that is inhibited by mutations that impair autophagosome formation in vivo. These results suggest that Atg2 acts as a lipid-transfer protein that supplies phospholipids for autophagosome formation. |
| 巻・号 | 26(4) |
| ページ | 281-288 |
| 公開日 | 2019-4-1 |
| DOI | 10.1038/s41594-019-0203-4 |
| PII | 10.1038/s41594-019-0203-4 |
| PMID | 30911189 |
| MeSH | Autophagosomes / metabolism* Autophagy / physiology Autophagy-Related Proteins / metabolism Carrier Proteins / metabolism Endoplasmic Reticulum / metabolism* Hydrophobic and Hydrophilic Interactions Liposomes / metabolism Phospholipids / metabolism Schizosaccharomyces / metabolism* |
| IF | 12.109 |
| 引用数 | 46 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 45 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | S. pombe entry clone SpFFH31A11 (SPW052411) |