論文 - 詳細
| RRC ID | 55035 |
|---|---|
| 著者 | Fujii S, Nagata K, Matsumoto S, Kohashi KI, Kikuchi A, Oda Y, Kiyoshima T, Wada N. |
| タイトル | Wnt/β-catenin signaling, which is activated in odontomas, reduces Sema3A expression to regulate odontogenic epithelial cell proliferation and tooth germ development. |
| ジャーナル | Sci Rep |
| Abstract |
Odontomas, developmental anomalies of tooth germ, frequently occur in familial adenomatous polyposis patients with activated Wnt/β-catenin signaling. However, roles of Wnt/β-catenin signaling in odontomas or odontogenic cells are unclear. Herein, we investigated β-catenin expression in odontomas and functions of Wnt/β-catenin signaling in tooth germ development. β-catenin frequently accumulated in nucleus and/or cellular cytoplasm of odontogenic epithelial cells in human odontoma specimens, immunohistochemically. Wnt/β-catenin signaling inhibited odontogenic epithelial cell proliferation in both cell line and tooth germ development, while inducing immature epithelial bud formation. We identified Semaphorin 3A (Sema3A) as a downstream molecule of Wnt/β-catenin signaling and showed that Wnt/β-catenin signaling-dependent reduction of Sema3A expression resulted in suppressed odontogenic epithelial cell proliferation. Sema3A expression is required in appropriate epithelial budding morphogenesis. These results suggest that Wnt/β-catenin signaling negatively regulates odontogenic epithelial cell proliferation and tooth germ development through decreased-Sema3A expression, and aberrant activation of Wnt/β-catenin signaling may associate with odontoma formation. |
| 巻・号 | 9(1) |
| ページ | 4257 |
| 公開日 | 2019-3-12 |
| DOI | 10.1038/s41598-019-39686-1 |
| PII | 10.1038/s41598-019-39686-1 |
| PMID | 30862786 |
| PMC | PMC6414619 |
| MeSH | Adolescent Animals Cell Line Cell Proliferation Child Child, Preschool DNA Mutational Analysis Down-Regulation / physiology Embryo, Mammalian Epithelial Cells / physiology Gene Knockdown Techniques Humans Immunohistochemistry Mice Odontogenesis / physiology* Odontoma / genetics Odontoma / pathology* Odontoma / surgery Primary Cell Culture RNA, Small Interfering / metabolism Semaphorin-3A / analysis Semaphorin-3A / genetics Semaphorin-3A / metabolism* Tooth Germ / cytology Tooth Germ / embryology* Wnt Signaling Pathway / physiology* Young Adult beta Catenin / analysis beta Catenin / genetics beta Catenin / metabolism |
| IF | 4.011 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |