RRC ID 55059
著者 Máthé I, Tóth E, Mentes A, Szabó A, Márialigeti K, Schumann P, Felföldi T.
タイトル A new Rhizobium species isolated from the water of a crater lake, description of Rhizobium aquaticum sp. nov.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel isolate, strain SA-276T, was isolated from the water of Lake St. Ana, a crater lake which is located in Romania. Phylogenetic analysis based on the 16S rRNA gene revealed that the new strain is a member of the family Rhizobiaceae, showing a high pairwise similarity value (97.65%) to Rhizobium tubonense CCBAU 85046T (= DSM 25379T), Rhizobium leguminosarum USDA 2370T (= LMG 14904T), Rhizobium anhuiense CCBAU 23252T and Rhizobium laguerreae FB206T. Cells of strain SA-276T were rod-shaped, motile, oxidase negative and weakly catalase positive. The predominant fatty acids were C18:1ω7c and cyclo C19:0ω8c, the major respiratory quinones were Q-10 and Q-9, and the main polar lipids were phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine. The G + C content of the genomic DNA of strain SA-276T was 60.8 mol%. The novel isolate can be distinguished from the closest related type strain R. tubonense DSM 25379T based on its broader substrate specificity and positive trypsin enzyme activity. On the basis of the phenotypic, chemotaxonomic and molecular data, strain SA-276T is considered to represent a new species, for which the name Rhizobium aquaticum sp. nov. is proposed. The type strain is SA-276T (= DSM 29780T = JCM 31760T).
巻・号 111(11)
ページ 2175-2183
公開日 2018-11-1
DOI 10.1007/s10482-018-1110-0
PII 10.1007/s10482-018-1110-0
PMID 29881978
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics DNA, Ribosomal / genetics Fatty Acids / chemistry Fatty Acids / metabolism Lakes / microbiology* Phylogeny Rhizobium / classification Rhizobium / genetics Rhizobium / isolation & purification* Rhizobium / metabolism
IF 1.934
引用数 3
リソース情報
一般微生物 JCM 31760