| RRC ID |
55063
|
| 著者 |
Ekimova GA, Fedorov DN, Tani A, Doronina NV, Trotsenko YA.
|
| タイトル |
Distribution of 1-aminocyclopropane-1-carboxylate deaminase and D-cysteine desulfhydrase genes among type species of the genus Methylobacterium.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
The presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase determines the ability of bacteria to increase the resistance of plants to various types of stress. The genes of ACC deaminase (acdS) and the closely related enzyme D-cysteine desulfhydrase (dcyD) were searched in type strains of various representatives of the genus Methylobacterium. Using PCR screening and in silico searching in the available complete genome sequences of type strains, the genes were found in 28 of 48 species of the genus. Phylogenetic analysis of amino acid sequences of proteins revealed two large groups of sequences of the AcdS protein and one of the DcyD protein. The distribution of these groups correlates well with the phylogenetic tree based on the sequences of the 16S rRNA genes, which apparently indicates a different evolutionary adaptation to association with plants in the representatives of these groups. For the first time for aerobic methylotrophs it was demonstrated that the gene dcyD encodes D-cysteine desulfhydrase by cloning and recombinant protein characterization.
|
| 巻・号 |
111(10)
|
| ページ |
1723-1734
|
| 公開日 |
2018-10-1
|
| DOI |
10.1007/s10482-018-1061-5
|
| PII |
10.1007/s10482-018-1061-5
|
| PMID |
29520528
|
| MeSH |
Carbon-Carbon Lyases / genetics*
Carbon-Carbon Lyases / metabolism
Cloning, Molecular
Cystathionine gamma-Lyase / genetics*
Cystathionine gamma-Lyase / metabolism
Genes, Bacterial
Methylobacterium / classification
Methylobacterium / enzymology
Methylobacterium / genetics*
Phylogeny
Plant Growth Regulators / metabolism
RNA, Ribosomal, 16S
Sequence Analysis, DNA
|
| IF |
1.934
|
| 引用数 |
3
|
| リソース情報 |
| 一般微生物 |
JCM 10891
JCM 10892 |
