RRC ID 55070
Author Jin L, Wu X, Ko SR, Jin FJ, Li T, Ahn CY, Oh HM, Lee HG.
Title Description of Hymenobacter daejeonensis sp. nov., isolated from grass soil, based on multilocus sequence analysis of the 16S rRNA gene, gyrB and tuf genes.
Journal Antonie Van Leeuwenhoek
Abstract A polyphasic taxonomic study was carried out on strains PB105T and PB108 isolated from a grass soil in Korea. The cells of the strains were Gram-stain negative, non-spore-forming, non-motile, and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of these strains with Bacteroidetes, which showed high pairwise sequence similarities with Hymenobacter algoricola VUG-A23aT (99.2%), Hymenobacter fastidiosus VUG-A124aT (97.4%), and Hymenobacter daecheongensis Dae14T (96.9%). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strains formed a clear phylogenetic lineage with the genus Hymenobacter. The major fatty acids were identified as C15:0 iso, C15:0 anteiso, C16:1 ω5c, C15:0 iso 3-OH, C17:0 iso 3-OH, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c/t), and summed feature 4 (C17:1 anteiso B and/or C17:1 iso I). The major cellular polar lipids were identified as phosphatidylethanolamine, an unidentified aminolipid, and two unidentified lipids. The respiratory quinone was identified as MK-7 and the genomic DNA G+C content was determined to be 64.5 mol% for strain PB105T and 64.1 mol% for strain PB108. DNA-DNA hybridization value of type strain PB105T with H. algoricola VUG-A23aT was 32.3% (reciprocal 39.2). Based on the combined genotypic and phenotypic data, we propose that strains PB105T and PB108 represent a novel species of the genus Hymenobacter, for which the name Hymenobacter daejeonensis sp. nov. is proposed. The type strain is PB105T (= KCTC 52579T = JCM 31885T).
Volume 111(12)
Pages 2283-2292
Published 2018-12-1
DOI 10.1007/s10482-018-1119-4
PII 10.1007/s10482-018-1119-4
PMID 29934694
MeSH Base Composition Cytophagaceae / classification Cytophagaceae / genetics Cytophagaceae / isolation & purification* Cytophagaceae / metabolism DNA, Bacterial / genetics Fatty Acids / chemistry Fatty Acids / metabolism Multilocus Sequence Typing Phylogeny Poaceae / growth & development RNA, Ribosomal, 16S / genetics Soil Microbiology*
IF 1.934
Times Cited 2
General Microbes JCM 31885 JCM 31886 JCM 27214 JCM 27227