| RRC ID |
55105
|
| 著者 |
Kawata M, Tsukamoto A, Isozaki R, Nobukawa S, Kawahara N, Akutsu S, Suzuki M, Asanuma N.
|
| タイトル |
Glucerabacter canisensis gen. nov., sp. nov., isolated from dog feces and its effect on the hydrolysis of plant glucosylceramide in the intestine of dogs.
|
| ジャーナル |
Arch Microbiol
|
| Abstract |
A Gram-positive, obligately anaerobic, oval-rod shaped, non-spore-forming, and non-pigmented bacterium, designated strain NATH-2371T (= JCM31739T = DSM105698T), was isolated from dog feces. Comparative 16S rRNA gene sequence analysis revealed that strain NATH-2371T belongs to Clostridium cluster XIVa, and the closest strains were Coprococcus comes ATCC 27758T (94.8% 16S rRNA gene sequence similarity) and Clostridium nexile DSM 1787T (94.0%). Strain NATH-2371T produced acetate, formate, and ethanol from glucose. Predominant cellular fatty acids are C16:0 and C16:0 DMA. On the basis of the phenotypic and genotypic differences, strain NATH-2371T represents a novel species in a new genus of the family Lachnospiraceae, for which the name Glucerabacter canisensis gen. nov., sp. nov., is proposed. This strain was found to efficiently hydrolyze plant glucosylceramide (GluCer). The abundance of strain NATH-2371T in dog feces was higher in young dogs than in old dogs. Incubation of dog fecal bacteria showed that GluCer-hydrolyzing activity decreased with the age of dogs. The cell number of strain NATH-2371T in dog feces appeared to be correlated with GluCer hydrolysis. Thus, this bacterium is likely to play a major role in GluCer hydrolysis in the dog intestine.
|
| 巻・号 |
200(3)
|
| ページ |
505-515
|
| 公開日 |
2018-4-1
|
| DOI |
10.1007/s00203-017-1463-1
|
| PII |
10.1007/s00203-017-1463-1
|
| PMID |
29204666
|
| MeSH |
Animals
Base Composition
Clostridium / genetics*
Clostridium / isolation & purification
Clostridium / metabolism
DNA, Bacterial / genetics
Dogs
Fatty Acids / analysis
Fatty Acids / chemistry
Feces / microbiology
Female
Glucosylceramides / metabolism*
Hydrolysis
Intestines / microbiology
Molecular Typing
Phylogeny
Plant Extracts / metabolism
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
1.642
|
| 引用数 |
1
|
| リソース情報 |
| 一般微生物 |
JCM 31739
JCM 14655
JCM 1395 |
