RRC ID 55109
著者 Nakatani A, Li X, Miyamoto J, Igarashi M, Watanabe H, Sutou A, Watanabe K, Motoyama T, Tachibana N, Kohno M, Inoue H, Kimura I.
タイトル Dietary mung bean protein reduces high-fat diet-induced weight gain by modulating host bile acid metabolism in a gut microbiota-dependent manner.
ジャーナル Biochem Biophys Res Commun
Abstract The 8-globulin-rich mung bean protein (MPI) suppresses hepatic lipogenesis in rodent models and reduces fasting plasma glucose and insulin levels in obese adults. However, its effects on mitigating high fat diet (HFD)-induced obesity and the mechanism underlying these effects remain to be elucidated. Herein, we examined the metabolic phenotype, intestinal bile acid (BA) pool, and gut microbiota of conventionally raised (CONV-R) male C57BL/6 mice and germ-free (GF) mice that were randomized to receive either regular HFD or HFD containing mung bean protein isolate (MPI) instead of the dairy protein present in regular HFD. MPI intake significantly reduced HFD-induced weight gain and adipose tissue accumulation, and attenuated hepatic steatosis. Enhancement in the secretion of intestinal glucagon-like peptide-1 (GLP-1) and an enlarged cecal and fecal BA pool of dramatically elevated secondary/primary BA ratio were observed in mice that had consumed MPI. These effects were abolished in GF mice, indicating that the effects were dependent upon the presence of the microbiota. As revealed by 16S rRNA gene sequence analysis, MPI intake also elicited dramatic changes in the gut microbiome, such as an expansion of taxa belonging to the phylum Bacteroidetes along with a reduced abundance of the Firmicutes.
巻・号 501(4)
ページ 955-961
公開日 2018-7-2
DOI 10.1016/j.bbrc.2018.05.090
PII S0006-291X(18)31153-7
PMID 29777704
MeSH Animals Bile Acids and Salts / metabolism* Cecum / metabolism Diet, High-Fat Dietary Proteins / pharmacology* Feces Gastrointestinal Microbiome / drug effects* Germ-Free Life Male Mice, Inbred C57BL Phenotype Plant Proteins / pharmacology* Vigna / chemistry* Weight Gain / drug effects*
IF 2.705
引用数 14
リソース情報
一般微生物 JCM 1395