論文 - 詳細
| RRC ID | 55111 |
|---|---|
| 著者 | Taguchi AT, Ohmori D, Dikanov SA, Iwasaki T. |
| タイトル | g-Tensor Directions in the Protein Structural Frame of Hyperthermophilic Archaeal Reduced Rieske-Type Ferredoxin Explored by 13C Pulsed Electron Paramagnetic Resonance. |
| ジャーナル | Biochemistry |
| Abstract |
Interpretation of magnetic resonance data in the context of structural and chemical biology requires prior knowledge of the g-tensor directions for paramagnetic metallo-cofactors with respect to the protein structural frame. Access to this information is often limited by the strict requirement of suitable protein crystals for single-crystal electron paramagnetic resonance (EPR) measurements or the reliance on protons (with ambiguous locations in crystal structures) near the paramagnetic metal site. Here we develop a novel pulsed EPR approach with selective 13Cβ-cysteine labeling of model [2Fe-2S] proteins to help bypass these problems. Analysis of the 13Cβ-cysteine hyperfine tensors reproduces the g-tensor of the Pseudomonas putida ISC-like [2Fe-2S] ferredoxin (FdxB). Its application to the hyperthermophilic archaeal Rieske-type [2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus, for which the single-crystal EPR approach was not feasible, supports the best-fit g x-, g z-, and g y-tensor directions of the reduced cluster as nearly along Fe-Fe, S-S, and the cluster plane normal, respectively. These approximate principal directions of the reduced ARF g-tensor, explored by 13C pulsed EPR, are less skewed from the cluster molecular axes and are largely consistent with those previously determined by single-crystal EPR for the cytochrome bc1-associated, reduced Rieske [2Fe-2S] center. This suggests the approximate g-tensor directions are conserved across the phylogenetically and functionally divergent Rieske-type [2Fe-2S] proteins. |
| 巻・号 | 57(28) |
| ページ | 4074-4082 |
| 公開日 | 2018-7-17 |
| DOI | 10.1021/acs.biochem.8b00438 |
| PMID | 29890072 |
| MeSH | Archaeal Proteins / chemistry* Bacterial Proteins / chemistry Carbon Isotopes / analysis Crystallography, X-Ray Cysteine / analysis Electron Spin Resonance Spectroscopy / methods* Ferredoxins / chemistry* Iron-Sulfur Proteins / chemistry Models, Molecular Protein Conformation Pseudomonas putida / chemistry Sulfolobus solfataricus / chemistry* |
| IF | 2.952 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 20004 |