RRC ID 55137
Author Ten LN, Elderiny N, Lee JJ, Lee SY, Park S, Lee DS, Kim MK, Jung HY.
Title Spirosoma harenae sp. nov., a Bacterium Isolated from a Sandy Beach.
Journal Curr. Microbiol.
Abstract A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-9T, was isolated from a sandy beach in Jeju Island, South Korea. The isolate was able to grow between 10 and 30 °C, pH 5-8, and in presence of 0-1% (w/v) NaCl. Based on 16S rRNA gene phylogenetic analysis, the novel strain was closely related to members of the genus Spirosoma (96.1-90.9% similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099T (96.1%). The G + C content of the genomic DNA of strain 15J8-9T was 45.1 mol%. The isolate contained menaquinone MK-7 as the predominant respiratory quinone, phosphatidylethanolamine as the major polar lipid, and summed feature 3 (C16:1 ω6c/C16:1 ω7c; 28.0%), C16:1 ω5c (23.4%), iso-C15:0 (13.5%), and C16:0 (11.5%) as the major fatty acids that supported the affiliation of strain 15J8-9T to the genus Spirosoma. The isolate could be differentiated clearly from recognized Spirosoma species on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain 15J8-9T is considered to represent a novel species of the genus the genus Spirosoma, for which the name Spirosoma harenae sp. nov. is proposed. The type strain is 15J8-9T (= KCTC 52030T = JCM 31993T).
Volume 75(2)
Pages 179-185
Published 2018-2
DOI 10.1007/s00284-017-1363-2
PII 10.1007/s00284-017-1363-2
PMID 29022066
MeSH Aerobiosis Base Composition Cluster Analysis Cytophagaceae / classification* Cytophagaceae / genetics Cytophagaceae / isolation & purification* Cytophagaceae / physiology Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Hydrogen-Ion Concentration Korea Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Soil Microbiology* Temperature Vitamin K 2 / analysis
IF 1.373
General Microbes JCM 31993