論文 - 詳細
| RRC ID | 55154 |
|---|---|
| 著者 | Wang Y, Sakka M, Yagi H, Kaneko S, Katsuzaki H, Kunitake E, Kimura T, Sakka K. |
| タイトル | Ruminiclostridium josui Abf62A-Axe6A: A tri-functional xylanolytic enzyme exhibiting α-l-arabinofuranosidase, endoxylanase, and acetylxylan esterase activities. |
| ジャーナル | Enzyme Microb Technol |
| Abstract |
Ruminiclostridium josui Abf62A-Axe6A is a modular enzyme comprising (in order from the N-terminus): an N-terminal signal peptide, a glycoside hydrolase family 62 (GH62) catalytic module, a family 6 carbohydrate binding module (CBM6), a dockerin module and an additional carbohydrate esterase family 6 catalytic module (CE6). In this study, three Abf62A-Axe6A derivatives were constructed, overexpressed in Escherichia coli, purified, and biochemically characterized: RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide; RjAbf62A-CBM6, containing the GH62 and CBM6 modules; and RjAxe6A, containing only CE6. RjAbf62A-Axe6A was highly active toward arabinoxylan and moderately active toward sugar beet arabinan, and released mainly arabinose. Analysis of the arabinoxylooligosaccharide hydrolysis products revealed that RjAbf62A-Axe6A released α-1,2- and α-1,3-linked arabinofuranose from both singly and doubly substituted xylosyl residues. Furthermore, RjAbf62A-Axe6A exhibited a weak activity toward linear 1,5-α-l arabinan and arabinooligosaccharides, indicating that it is capable of cleaving α-1,5-linkage. Surprisingly, RjAbf62A-Axe6A also demonstrated an endoxylanase activity toward birchwood and beechwood xylans and xylooligosaccharides. Although RjAbf62A-CBM6 exhibited a similar substrate specificity to RjAbf62A-Axe6A, RjAbf62A-CBM6 showed lower activities toward soluble arabinoxylans, birchwood and beechwood xylans and arabinoxylooligosaccharides but not toward insoluble arabinoxylan. RjAbf62A-Axe6A is the first reported GH62 enzyme with α-l-arabinofuranosidase and endoxylanase activities. Although both RjAbf62A-Axe6A and RjAxe6A had acetylxylan esterase activities, RjAbf62A-Axe6 exhibited a higher activity toward insoluble wheat arabinoxylan compared with RjAxe6. |
| 巻・号 | 117 |
| ページ | 1-8 |
| 公開日 | 2018-10-1 |
| DOI | 10.1016/j.enzmictec.2018.05.016 |
| PII | S0141-0229(18)30224-2 |
| PMID | 30037546 |
| MeSH | Acetylesterase / genetics Acetylesterase / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism Catalytic Domain Clostridiales / enzymology* Endo-1,4-beta Xylanases / genetics Endo-1,4-beta Xylanases / metabolism* Glycoside Hydrolases / genetics Glycoside Hydrolases / metabolism* Hydrolysis Recombinant Proteins / genetics Recombinant Proteins / metabolism Substrate Specificity Xylans / metabolism* |
| IF | 2.932 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 17888 |