| RRC ID |
55425
|
| Author |
Lam KL, Lin S, Liu C, Wu X, Tang S, Kwan HS, Cheung PC.
|
| Title |
Low-Cost Method Generating In Situ Anaerobic Conditions on a 96-Well Plate for Microbial Fermentation in Food Research.
|
| Journal |
J Agric Food Chem
|
| Abstract |
Commercial tools and instruments have been developed for a screening study of microbial fermentation, but they are expensive and mostly confined to aerobic fermentation only. There is little development on the generation of anaerobic conditions directly on a 96-well plate. This report proposed a simple and versatile microbial fermentation system known as OVAMO that makes use of Oxyrase, vacuum, and mineral oil to generate an in situ anaerobic environment on a 96-well plate for at least 48 h. The practicality of OVAMO in anaerobic fermentation experiments used for functional food research was validated by a prebiotic screening study of different carbohydrates by Bifidobacterium longum subsp. infantis. The OVAMO system provides a less expensive but effective way to conduct a microbial fermentation screening study that requires anaerobic conditions without the need for atmospheric control by external devices.
|
| Volume |
66(44)
|
| Pages |
11839-11845
|
| Published |
2018-11-7
|
| DOI |
10.1021/acs.jafc.8b04888
|
| PMID |
30277075
|
| MeSH |
Anaerobiosis
Bifidobacterium / growth & development
Bifidobacterium / metabolism*
Fermentation
Food Microbiology / economics
Food Microbiology / instrumentation
Food Microbiology / methods*
Oxygen / analysis
Oxygen / metabolism
Prebiotics / analysis
|
| IF |
3.571
|
| Times Cited |
1
|
| Resource |
| General Microbes |
JCM 1222 |
