RRC ID 55466
著者 Song MM, Veeranagouda Y, Ganzorig M, Lee K.
タイトル Circular pellicles formed by Pseudomonas alkylphenolica KL28 are a sophisticated architecture principally designed by matrix substance.
ジャーナル J Microbiol
Abstract The colonization of liquid surfaces as floating biofilms or pellicles is a bacterial adaptation to optimally occupy the airliquid (A-L) niche. In aerobic heterotrophs, pellicle formation is beneficial for the utilization of O2 and nonpolar organic compounds. Pseudomonas alkylphenolica KL28, an alkylphenol degrader, forms flat circular pellicles that are 0.3-0.5 mm in diameter. In this study, we first monitored the pellicle developmental patterns of multicellular organization from the initial settlement stage. The pellicles developed by clonal growth and mutants for flagella and pilus formation established normal pellicles. In contrast, the mutants of an epm gene cluster for biosynthesis of alginate-like polymer were incompetent in cell alignment for initial two-dimensional (2D) pellicle growth, suggesting the role of the Epm polymer as a structural scaffold for pellicle biofilms. Microscopic observation revealed that the initial 2D growth transited to multilayers by an accumulated self-produced extracellular polymeric substance that may exert a constraint force. Electron microscopy and confocal laser scanning microscopy revealed that the fully matured pellicle structures were densly packed with matrix-encased cells displaying distinct arrangements. The cells on the surface of the pellicle were relatively flat, and those inside were longitudinally cross-packed. The extracellular polysaccharide stained by Congo red was denser on the pellicle rim and a thin film was observed in the open spaces, indicative of its role in pellicle flotation. Our results demonstrate that P. alkylphenolica KL28 coordinately dictates the cell arrangements of pellicle biofilms by the controlled growth of constituent cells that accumulate extracellular polymeric substances.
巻・号 56(11)
ページ 790-797
公開日 2018-11-1
DOI 10.1007/s12275-018-8252-7
PII 10.1007/s12275-018-8252-7
PMID 30353464
MeSH Alginates / metabolism Bacterial Proteins / genetics Biofilms / growth & development* Extracellular Matrix / chemistry Extracellular Matrix / metabolism* Fimbriae, Bacterial / genetics Fimbriae, Bacterial / metabolism Flagella / genetics Flagella / metabolism Genes, Bacterial / genetics Multigene Family Mutation Polysaccharides, Bacterial / chemistry Polysaccharides, Bacterial / metabolism* Pseudomonas / cytology Pseudomonas / genetics Pseudomonas / growth & development Pseudomonas / metabolism*
IF 2.319
引用数 0
リソース情報
一般微生物 JCM 16553