Reference - Detail
| RRC ID | 55481 |
|---|---|
| Author | Ootsuka M, Nishizawa T, Hasegawa M, Kurusu Y, Ohta H. |
| Title | Comparative Analysis of the Genetic Basis of Branched Nonylphenol Degradation by Sphingobium amiense DSM 16289T and Sphingobium cloacae JCM 10874T. |
| Journal | Microbes Environ |
| Abstract |
Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae JCM 10874T. An opdA homolog (opdADSM16289) encoding BNP degradation activity was identified in DSM 16289T, in contrast with JCM 10874T, possessing both the opdA homolog and nmoA. The degradation profile of different BNP isomers was examined by Escherichia coli transformants harboring opdADSM16289, opdAJCM10874, and nmoAJCM10874 to characterize and compare the expression activities of these genes. |
| Volume | 33(4) |
| Pages | 450-454 |
| Published | 2018-12-28 |
| DOI | 10.1264/jsme2.ME18077 |
| PMID | 30518740 |
| PMC | PMC6308004 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism* Escherichia coli / genetics Escherichia coli / metabolism Genes, Bacterial / genetics Genome, Bacterial / genetics* Phenols / metabolism* Plasmids Recombinant Proteins / genetics Recombinant Proteins / metabolism Sphingomonadaceae / genetics* Sphingomonadaceae / metabolism Substrate Specificity |
| IF | 2.575 |
| Times Cited | 2 |
| Resource | |
| General Microbes | JCM 10874 |