論文 - 詳細
| RRC ID | 55487 |
|---|---|
| 著者 | Sun L, Lu Z, Li J, Sun F, Huang R. |
| タイトル | Comparative genomics and transcriptome analysis of Lactobacillus rhamnosus ATCC 11443 and the mutant strain SCT-10-10-60 with enhanced L-lactic acid production capacity. |
| ジャーナル | Mol Genet Genomics |
| Abstract |
Mechanisms for high L-lactic acid production remain unclear in many bacteria. Lactobacillus rhamnosus SCT-10-10-60 was previously obtained from L. rhamnosus ATCC 11443 via mutagenesis and showed improved L-lactic acid production. In this study, the genomes of strains SCT-10-10-60 and ATCC 11443 were sequenced. Both genomes are a circular chromosome, 2.99 Mb in length with a GC content of approximately 46.8%. Eight split genes were identified in strain SCT-10-10-60, including two LytR family transcriptional regulators, two Rex redox-sensing transcriptional repressors, and four ABC transporters. In total, 60 significantly up-regulated genes (log2fold-change ≥ 2) and 39 significantly down-regulated genes (log2fold-change ≤ - 2) were identified by a transcriptome comparison between strains SCT-10-10-60 and ATCC 11443. KEGG pathway enrichment analysis revealed that "pyruvate metabolism" was significantly different (P < 0.05) between the two strains. The split genes and the differentially expressed genes involved in the "pyruvate metabolism" pathway are probably responsible for the increased L-lactic acid production by SCT-10-10-60. The genome and transcriptome sequencing information and comparison of SCT-10-10-60 with ATCC 11443 provide insights into the anabolism of L-lactic acid and a reference for improving L-lactic acid production using genetic engineering. |
| 巻・号 | 293(1) |
| ページ | 265-276 |
| 公開日 | 2018-2-1 |
| DOI | 10.1007/s00438-017-1379-0 |
| PII | 10.1007/s00438-017-1379-0 |
| PMID | 29159508 |
| MeSH | Gene Expression Regulation, Bacterial Genome, Bacterial Genomics* Lactic Acid / biosynthesis* Lacticaseibacillus rhamnosus / genetics* Lacticaseibacillus rhamnosus / metabolism Metabolic Engineering Mutation Pyruvic Acid / metabolism Transcriptome / genetics* |
| IF | 2.879 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1553 |