RRC ID 55489
Author Matsuda Y, Cho O, Sugita T, Ogishima D, Takeda S.
Title Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells.
Journal Mycopathologia
Abstract PURPOSE:Vulvovaginal candidiasis (VVC) is a common superficial infection of the vaginal mucous membranes caused by the fungus Candida albicans. The aim of this study was to assess the mechanisms underlying the inhibitory effects of the culture supernatants of Lactobacillus gasseri and L. crispatus, the predominant microbiota in Asian healthy women, on C. albicans biofilm formation. The inhibition of C. albicans adhesion to HeLa cells by Lactobacillus culture supernatant was also investigated.
METHODS:Candida albicans biofilm was formed on polystyrene flat-bottomed 96-well plates, and the inhibitory effects on the initial colonization and maturation phases were determined using the XTT reduction assay. The expression levels of biofilm formation-associated genes (HWP1, ECE1, ALS3, BCR1, EFG1, TEC1, and CPH1) were determined by reverse transcription quantitative polymerase chain reaction. The inhibition of C. albicans adhesion to HeLa cells by Lactobacillus culture supernatant was evaluated by enumerating viable C. albicans cells.
RESULTS:The culture supernatants of both Lactobacillus species inhibited the initial colonization and maturation of C. albicans biofilm. The expression levels of all biofilm formation-related genes were downregulated in the presence of Lactobacillus culture supernatant. The culture supernatant also inhibited C. albicans adhesion to HeLa cells.
CONCLUSION:The culture supernatants of L. gasseri and L. crispatus inhibited C. albicans biofilm formation by downregulating biofilm formation-related genes and C. albicans adhesion to HeLa cells. These findings support the notion that Lactobacillus metabolites may be useful alternatives to antifungal drugs for the management of VVC.
Volume 183(4)
Pages 691-700
Published 2018-8-1
DOI 10.1007/s11046-018-0259-4
PII 10.1007/s11046-018-0259-4
PMID 29603066
MeSH Anti-Infective Agents / pharmacology* Biofilms / drug effects* Biofilms / growth & development Candida albicans / drug effects* Candida albicans / physiology Cell Adhesion / drug effects* Culture Media / chemistry* Female Formazans / analysis HeLa Cells Humans Lactobacillus crispatus / growth & development Lactobacillus crispatus / metabolism* Lactobacillus gasseri / growth & development Lactobacillus gasseri / metabolism* Microbial Viability / drug effects Staining and Labeling
IF 2.278
Times Cited 6
Resource
General Microbes JCM 1131 JCM 1185