| RRC ID |
55490
|
| 著者 |
Piri F, Zarei Mahmoudabadi A, Ronagh A, Ahmadi B, Makimura K, Rezaei-Matehkolaei A.
|
| タイトル |
Assessment of a pan-dermatophyte nested-PCR compared with conventional methods for direct detection and identification of dermatophytosis agents in animals.
|
| ジャーナル |
Mycoses
|
| Abstract |
Conventional direct microscopy with potassium hydroxide (KOH) and culture were found to lack the ability to establish a fast and specific diagnosis of dermatophytosis. A pan-dermatophyte nested-PCR assay was developed using a novel primer pair targeting the translation elongation factor 1-α (Tef-1α) sequences for direct detection and identification of most veterinary relevant dermatophytes in animal samples suspected to dermatophytosis. A total of 140 animal skin and hair samples were subjected to direct microscopy, culture, and ITS-RFLP/ITS-sequencing of culture isolates for the detection and identification of dermatophytosis agents. Nested-PCR sequencing was performed on all the extracted DNAs using a commercial kit after dissolving the specimens by mechanical beating. Nested-PCR was positive in 90% of samples, followed by direct microscopy (85.7%) and culture (75%). The degree of agreement between nested-PCR and direct microscopy (94.4%) was higher than with culture (83.3%). In 105 culture-positive cases, the measures of agreement for the identification of dermatophytosis agents were as follows: 100% between nested-PCR sequencing and ITS-RFLP/ITS-sequencing and 63.8% between nested-PCR sequencing and culture. The developed nested-PCR was faster as well as more sensitive and specific than conventional methods for detection and identification of dermatophytes in clinical samples, which was particularly suitable for epidemiological studies.
|
| 巻・号 |
61(11)
|
| ページ |
837-844
|
| 公開日 |
2018-11-1
|
| DOI |
10.1111/myc.12821
|
| PMID |
29944743
|
| MeSH |
Animal Diseases / diagnosis
Animal Diseases / microbiology*
Animals
Arthrodermataceae / classification
Arthrodermataceae / genetics
Arthrodermataceae / isolation & purification*
Cats
Cattle
DNA, Fungal / genetics
Dogs
Goats
Horses
Microscopy / methods*
Microscopy / veterinary
Polymerase Chain Reaction / methods*
Polymerase Chain Reaction / veterinary
Sensitivity and Specificity
Sheep
Tinea / diagnosis
Tinea / microbiology
Tinea / veterinary*
|
| IF |
3.065
|
| 引用数 |
3
|
| リソース情報 |
| 一般微生物 |
JCM 1885
JCM 1907
JCM 2061 |
