RRC ID 55532
Author Kunthiphun S, Chokreansukchai P, Hondee P, Tanasupawat S, Savarajara A.
Title Diversity and characterization of cultivable oleaginous yeasts isolated from mangrove forests.
Journal World J Microbiol Biotechnol
Abstract A total of 198 yeasts were isolated from 140 samples collected from 7 mangrove forests in 4 provinces of Thailand, and were found to belong to 30 genera, 45 described species and at least 12 undescribed species based on their 26S rRNA (D1/D2 domain) gene sequence. The most prevalent species was Candida tropicalis, followed by Candida pseudolambica and Rhodosporidium paludigena. Lipid accumulation, as determined by Nile red staining, of the isolated yeasts revealed that 69 and 18 strains were positive and strongly positive, respectively, while quantitative analysis of the intracellular lipid accumulated in the latter indicated that 10 of these strains, Pseudozyma tsukubaensis (YWT7-2 and YWT7-3), Rhodotorula sphaerocarpa (YWW6-1 and SFL14-1SF), Saitozyma podzolica (YWT1-1, NS3-3 and NS10-2), Prototheca zopfii var. hydrocarbonea OMS6-1 and Prototheca sp. (YMTW3-1 and YMTS5-2), were oleaginous. In this study we found that under nitrogen depletion condition (155 C/N ratio) Pseudozyma tsukubaensis YWT7-2 accumulated the highest level of intracellular lipid at 32.4% (w/w, dry cell weight), with a broadly similar fatty acid composition to that in palm oil.
Volume 34(9)
Pages 125
Published 2018-8-6
DOI 10.1007/s11274-018-2507-7
PII 10.1007/s11274-018-2507-7
PMID 30083778
MeSH Base Sequence Biodiversity* DNA, Fungal Fatty Acids / analysis Lipids / analysis Nitrogen / metabolism Phylogeny* RNA, Ribosomal / genetics Species Specificity Thailand Water Microbiology Wetlands* Yeasts / classification* Yeasts / genetics Yeasts / isolation & purification* Yeasts / physiology
IF 2.1
Times Cited 4
General Microbes JCM 5995