RRC ID 5569
Author Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A.
Title Sex-reversed somatic cell cloning in the mouse.
Journal J. Reprod. Dev.
Abstract Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species.
Volume 55(5)
Pages 566-9
Published 2009-10
PII JST.JSTAGE/jrd/09-099E
PMID 19602850
MeSH Age Factors Animals Cloning, Organism* Disorders of Sex Development* Embryo Transfer Female Genitalia, Female Genitalia, Male Karyotyping Male Mice Nuclear Transfer Techniques* Reproduction Sertoli Cells / cytology* Sertoli Cells / physiology
IF 1.5
Times Cited 4