Reference - Detail
| RRC ID | 5569 |
|---|---|
| Author | Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A. |
| Title | Sex-reversed somatic cell cloning in the mouse. |
| Journal | J Reprod Dev |
| Abstract |
Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species. |
| Volume | 55(5) |
| Pages | 566-9 |
| Published | 2009-10-1 |
| DOI | 10.1262/jrd.09-099e |
| PII | JST.JSTAGE/jrd/09-099E |
| PMID | 19602850 |
| MeSH | Age Factors Animals Cloning, Organism* Disorders of Sex Development* Embryo Transfer Female Genitalia, Female Genitalia, Male Karyotyping Male Mice Nuclear Transfer Techniques* Reproduction Sertoli Cells / cytology* Sertoli Cells / physiology |
| IF | 1.735 |
| Times Cited | 6 |
| WOS Category | AGRICULTURE, DAIRY & ANIMAL SCIENCE REPRODUCTIVE BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Mice | RBRC01260 |