| RRC ID |
5569
|
| 著者 |
Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A.
|
| タイトル |
Sex-reversed somatic cell cloning in the mouse.
|
| ジャーナル |
J Reprod Dev
|
| Abstract |
Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species.
|
| 巻・号 |
55(5)
|
| ページ |
566-9
|
| 公開日 |
2009-10-1
|
| DOI |
10.1262/jrd.09-099e
|
| PII |
JST.JSTAGE/jrd/09-099E
|
| PMID |
19602850
|
| MeSH |
Age Factors
Animals
Cloning, Organism*
Disorders of Sex Development*
Embryo Transfer
Female
Genitalia, Female
Genitalia, Male
Karyotyping
Male
Mice
Nuclear Transfer Techniques*
Reproduction
Sertoli Cells / cytology*
Sertoli Cells / physiology
|
| IF |
1.735
|
| 引用数 |
6
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC01260 |
