Reference - Detail
| RRC ID | 55707 |
|---|---|
| Author | Nagasawa K, Ishida M, Octavera A, Kusano K, Kezuka F, Kitano T, Yoshiura Y, Yoshizaki G. |
| Title | Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation†. |
| Journal | Biol Reprod |
| Abstract |
A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP). We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (-/-)) recovered their fertility and produced only donor-derived fshr (-) X eggs. Natural mating between these females and fshr (-/-) sex-reversed XX males successfully produced large numbers of sterile fshr (-/-) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established. |
| Volume | 100(2) |
| Pages | 535-546 |
| Published | 2019-2-1 |
| DOI | 10.1093/biolre/ioy204 |
| PII | 5106316 |
| PMID | 30252024 |
| MeSH | Animals Aromatase Inhibitors / pharmacology Female Gene Deletion Gene Expression Regulation / drug effects Gene Expression Regulation / genetics Genotype Hybridization, Genetic Infertility / genetics Infertility / veterinary* Male Oryzias / genetics* Oryzias / physiology* Receptors, FSH / genetics* Spermatogonia / physiology* |
| IF | 2.96 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Medaka | hi-medaka (MT835) |