論文 - 詳細
| RRC ID | 55713 |
|---|---|
| 著者 | Ito K, Kidokoro K, Katsuma S, Sezutsu H, Uchino K, Kobayashi I, Tamura T, Yamamoto K, Mita K, Shimada T, Kadono-Okuda K. |
| タイトル | A single amino acid substitution in the Bombyx-specific mucin-like membrane protein causes resistance to Bombyx mori densovirus. |
| ジャーナル | Sci Rep |
| Abstract |
Bombyx mori densovirus type 1 (BmDV) is a pathogen that causes flacherie disease in the silkworm. The absolute nonsusceptibility to BmDV among certain silkworm strains is determined independently by two genes, nsd-1 and Nid-1. However, neither of these genes has been molecularly identified to date. Here, we isolated the nsd-1 gene by positional cloning and characterized the properties of its product, NSD-1. Sequence and biochemical analyses revealed that this gene encodes a Bombyx-specific mucin-like glycoprotein with a single transmembrane domain. The NSD-1 protein was specifically expressed in the larval midgut epithelium, the known infection site of BmDV. Sequence analysis of the nsd-1 gene from 13 resistant and 12 susceptible strains suggested that a specific arginine residue in the extracellular tail of the NSD-1 protein was common among susceptible strains. Germline transformation of the susceptible-type nsd-1 (with a single nucleotide substitution) conferred partial susceptibility to resistant larvae, indicating that the + nsd-1 gene is required for the susceptibility of B. mori larvae to BmDV and the susceptibility is solely a result of the substitution of a single amino acid with arginine. Taken together, our results provide striking evidence that a novel membrane-bound mucin-like protein functions as a cell-surface receptor for a densovirus. |
| 巻・号 | 8(1) |
| ページ | 7430 |
| 公開日 | 2018-5-9 |
| DOI | 10.1038/s41598-018-25388-7 |
| PII | 10.1038/s41598-018-25388-7 |
| PMID | 29743532 |
| PMC | PMC5943349 |
| MeSH | Amino Acid Substitution* Animals Bombyx / metabolism Bombyx / physiology* Bombyx / virology* Cloning, Molecular Insect Proteins / genetics Insect Proteins / metabolism Insect Viruses / physiology* Membrane Proteins / genetics* Membrane Proteins / metabolism* Mucins / metabolism* Protein Transport Species Specificity Transformation, Genetic |
| IF | 4.011 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p50T |