RRC ID 55723
Author Morifuji Y, Xu J, Karasaki N, Iiyama K, Morokuma D, Hino M, Masuda A, Yano T, Mon H, Kusakabe T, Lee JM.
Title Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm-Baculovirus Expression System.
Journal Mol. Biotechnol.
Abstract Human α1-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α1-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). In this study, the baculovirus expression vector system using silkworm larvae as host was employed and a large amount of highly active AAT was recovered from the silkworm serum (~ 15 mg/10 ml) with high purity. Both the enzymatic activity and stability of purified rAAT were comparable with those of commercial product. Our results provide an alternative method for mass production of the active rAAT in pharmaceutical use.
Volume 60(12)
Pages 924-934
Published 2018-12
DOI 10.1007/s12033-018-0127-y
PII 10.1007/s12033-018-0127-y
PMID 30302632
MeSH Animals Baculoviridae / genetics* Bombyx / genetics* Bombyx / metabolism Cell Line Cloning, Molecular Humans Larva / genetics Larva / metabolism Recombinant Proteins / chemistry* Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism alpha 1-Antitrypsin / chemistry* alpha 1-Antitrypsin / genetics* alpha 1-Antitrypsin / isolation & purification alpha 1-Antitrypsin / metabolism
IF 1.815
Resource
Silkworms f38