論文 - 詳細
| RRC ID | 55723 |
|---|---|
| 著者 | Morifuji Y, Xu J, Karasaki N, Iiyama K, Morokuma D, Hino M, Masuda A, Yano T, Mon H, Kusakabe T, Lee JM. |
| タイトル | Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm-Baculovirus Expression System. |
| ジャーナル | Mol Biotechnol |
| Abstract |
Human α1-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α1-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). In this study, the baculovirus expression vector system using silkworm larvae as host was employed and a large amount of highly active AAT was recovered from the silkworm serum (~ 15 mg/10 ml) with high purity. Both the enzymatic activity and stability of purified rAAT were comparable with those of commercial product. Our results provide an alternative method for mass production of the active rAAT in pharmaceutical use. |
| 巻・号 | 60(12) |
| ページ | 924-934 |
| 公開日 | 2018-12-1 |
| DOI | 10.1007/s12033-018-0127-y |
| PII | 10.1007/s12033-018-0127-y |
| PMID | 30302632 |
| MeSH | Animals Baculoviridae / genetics* Bombyx / genetics* Bombyx / metabolism Cell Line Cloning, Molecular Humans Larva / genetics Larva / metabolism Recombinant Proteins / chemistry* Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism alpha 1-Antitrypsin / chemistry* alpha 1-Antitrypsin / genetics* alpha 1-Antitrypsin / isolation & purification alpha 1-Antitrypsin / metabolism |
| IF | 1.712 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | f38 |