RRC ID 55727
著者 Uno T, Ozakiya Y, Furutani M, Sakamoto K, Uno Y, Kajiwara H, Kanamaru K, Mizoguchi A.
タイトル Functional characterization of insect-specific RabX6 of Bombyx mori.
ジャーナル Histochem Cell Biol
Abstract Rab proteins are low-molecular weight (20-25 kDa) monomeric GTPases that are central to the control and regulation of vesicle trafficking. RabX6 is an insect-specific Rab protein that has no close homolog in vertebrates. However, little information about insect-specific Rab proteins is available. In this study, RabX6 was expressed in Escherichia coli and subsequently purified. Antibodies against Bombyx mori RabX6 were produced in rabbits and rats for western immunoblotting and immunohistochemistry. Western blotting of testis tissues revealed two bands, at positions corresponding to a molecular weight of approximately 26 kDa. RabX6-like immunohistochemical reactivity (RabX6-ir) was identified at the face of the testis, not in the spermatogonia, and was specifically detected at a pair of tritocerebral cells of the male brain. Furthermore, RNA interference of RabX6 was shown to decrease testicular growth. These findings suggest that RabX6 is involved in the regulation of testicular growth and male-specific neuropeptide secretion in the brain of B. mori.
巻・号 151(2)
ページ 187-198
公開日 2019-2-1
DOI 10.1007/s00418-018-1710-9
PII 10.1007/s00418-018-1710-9
PMID 30132061
MeSH Animals Bombyx / chemistry Bombyx / metabolism* Male Testis / growth & development Testis / metabolism rab GTP-Binding Proteins / analysis rab GTP-Binding Proteins / metabolism*
IF 2.64
引用数 1
リソース情報
カイコ p50