RRC ID |
5573
|
著者 |
Weidenfeld-Baranboim K, Hasin T, Darlyuk I, Heinrich R, Elhanani O, Pan J, Yokoyama KK, Aronheim A.
|
タイトル |
The ubiquitously expressed bZIP inhibitor, JDP2, suppresses the transcription of its homologue immediate early gene counterpart, ATF3.
|
ジャーナル |
Nucleic Acids Res
|
Abstract |
JDP2 is a ubiquitously expressed bZIP repressor protein. JDP2 binds TPA response element and cyclic AMP response element located within various promoters. JDP2 displays a high degree of homology to the immediate early gene ATF3. ATF3 plays a crucial role in the cellular adaptive response to multiple stress insults as well as growth stimuli. We have identified ATF3 as a potential target gene for JDP2 repression. JDP2 regulates the ATF3 promoter potentially through binding to both the consensus ATF/CRE site and a non-consensus ATF3 auto-repression DNA-binding element. Expression of ATF3 protein in wild-type mouse embryo fibroblast (MEF) cells is below the detectable levels, whereas, JDP2 disrupted MEF cells display noticeable level of ATF3 protein. Following either serum or ER stress stimulation, ATF3 expression is potentiated in JDP2-KO fibroblast cells as compared with wild-type cells. Mice with either JDP2 over-expression or JDP2 disruption display undetectable level of ATF3 protein. However, ATF3 induction in response to either growth or stress signals is dependent on JDP2 expression level. ATF3 induction is attenuated in JDP2 over-expressing mice whereas is potentiated in JDP2-KO mice as compared with the corresponding wild-type mice. Collectively, the data presented strongly suggest that JDP2 plays a role in the determination of the ATF3 adaptive cellular threshold response to different stress insults and growth stimuli.
|
巻・号 |
37(7)
|
ページ |
2194-203
|
公開日 |
2009-4-1
|
DOI |
10.1093/nar/gkp083
|
PII |
gkp083
|
PMID |
19233874
|
PMC |
PMC2673429
|
MeSH |
Activating Transcription Factor 3 / genetics*
Activating Transcription Factor 3 / metabolism
Angiotensin II / pharmacology
Animals
Binding Sites
Cell Line
Gene Expression Regulation*
Heart / drug effects
Humans
Isoproterenol / pharmacology
Liver / drug effects
Liver / metabolism
Mice
Mice, Knockout
Mice, Transgenic
Myocardium / metabolism
Promoter Regions, Genetic
Repressor Proteins / genetics
Repressor Proteins / metabolism*
Transcription, Genetic
|
IF |
11.502
|
引用数 |
33
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
実験動物マウス |
RBRC00861 |