Reference - Detail
| RRC ID | 55744 |
|---|---|
| Author | Tainaka K, Murakami TC, Susaki EA, Shimizu C, Saito R, Takahashi K, Hayashi-Takagi A, Sekiya H, Arima Y, Nojima S, Ikemura M, Ushiku T, Shimizu Y, Murakami M, Tanaka KF, Iino M, Kasai H, Sasaoka T, Kobayashi K, Miyazono K, Morii E, Isa T, Fukayama M, Kakita A, Ueda HR. |
| Title | Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents. |
| Journal | Cell Rep |
| Abstract |
We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1,600 chemicals were screened by a high-throughput evaluation system for each chemical process. The chemical profiling revealed important chemical factors: salt-free amine with high octanol/water partition-coefficient (logP) for delipidation, N-alkylimidazole for decoloring, aromatic amide for RI matching, and protonation of phosphate ion for decalcification. The strategic integration of optimal chemical cocktails provided a series of CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) protocols, which efficiently clear mouse organs, mouse body including bone, and even large primate and human tissues. The updated CUBIC protocols are scalable and reproducible, and they enable three-dimensional imaging of the mammalian body and large primate and human tissues. This strategy represents a future paradigm for the rational design of hydrophilic clearing cocktails that can be used for large tissues. |
| Volume | 24(8) |
| Pages | 2196-2210.e9 |
| Published | 2018-8-21 |
| DOI | 10.1016/j.celrep.2018.07.056 |
| PII | S2211-1247(18)31159-8 |
| PMID | 30134179 |
| MeSH | Humans Hydrophobic and Hydrophilic Interactions Indicators and Reagents / chemistry* |
| IF | 7.815 |
| Times Cited | 34 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 62 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Japanese macaques | |