論文 - 詳細
RRC ID | 55771 |
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著者 | Nishishita N, Takenaka C, Fusaki N, Kawamata S. |
タイトル | Generation of human induced pluripotent stem cells from cord blood cells. |
ジャーナル | J Stem Cells |
Abstract |
We report that iPS cells can be safely and effectively generated from fresh human cord blood (CB) cells with Sendai virus (SeV) vector carrying reprogramming factors OCT3/4, SOX2, KLF4, and c-MYC. The SeV vector is a single strand RNA virus having no DNA phase, and selectively infects the freshly isolated CD34+ CD45low+ fraction of CB cells corresponding to hematopoietic progenitors. Approximately twenty ES cell-like colonies emerged from 1 x 104 freshly isolated CD34+ CB cells around 18 days after SeV infection and were selected for passage to reduce the frequency of the remaining SeV-infected cells. The complete elimination of viral constructs was confirmed after several passages by immunostaining with monoclonal antibody against hemagglutinin-neuraminidase (HN) and by RT-PCR analysis. Five ES cell-like clones were selected to examine their in vitro potential for three germ layer differentiation and their capacity for teratoma formation. Generation of non-integrating Sendai virus (SeV) iPS cells from CB cells may be an important step to provide allogeneic iPS cell-derived therapy in the future. |
巻・号 | 6(3) |
ページ | 101-8 |
公開日 | 2011-1-1 |
PMID | 23264996 |
MeSH | Antigens, CD34 / metabolism Biomarkers / metabolism Cell Differentiation Cell Proliferation Cell Separation Cells, Cultured Cellular Reprogramming Coculture Techniques Feeder Cells Fetal Blood / cytology* Gene Expression Profiling / methods Gene Expression Regulation, Developmental Genetic Vectors Hematopoietic Stem Cells / immunology Hematopoietic Stem Cells / metabolism Hematopoietic Stem Cells / physiology* Humans Induced Pluripotent Stem Cells / immunology Induced Pluripotent Stem Cells / metabolism Induced Pluripotent Stem Cells / physiology* Karyotyping Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / genetics Kruppel-Like Transcription Factors / metabolism Octamer Transcription Factor-3 / genetics Octamer Transcription Factor-3 / metabolism Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / metabolism Sendai virus / genetics Time Factors Transfection |
IF | 1.94 |
リソース情報 | |
研究用ヒト臍帯血幹細胞 |