| RRC ID |
55785
|
| 著者 |
Nishishita N, Ijiri H, Takenaka C, Kobayashi K, Goto K, Kotani E, Itoh T, Mori H, Kawamata S.
|
| タイトル |
The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells.
|
| ジャーナル |
Biomaterials
|
| Abstract |
Human leukemia inhibitory factor (LIF) was immobilized into insect virus-derived microcrystals (polyhedra) to generate LIF polyhedra (LIF-PH) that can slowly release LIF into embryonic stem (ES) cell culture media and thus maintain ES cells in an undifferentiated state. Assays of the biological activities of LIF-PH indicated that a single addition of LIF-PH to the ES cell culture medium can support the proliferation of mouse ES and induced pluripotent stem (iPS) cells continuously for 14 days, and suggest that LIF-PH can be successfully used in the place of a periodic addition of recombinant LIF to the media every 2-3 days. The release of LIF protein from LIF-PH was determined by enzyme-linked immunosorbent assay (ELISA). Maintenance of undifferentiated state of mouse ES and iPS cells cultured with LIF-PH was determined by the detection of pluripotency-related biomarkers Oct3/4 and stage-specific embryonic antigen-1 (SSEA-1) through immunostaining and measurement of alkaline phosphatase activity. In this paper, we propose a closed culture system for mass production of ES and iPS cells that utilize a slow-releasing agent of LIF.
|
| 巻・号 |
32(14)
|
| ページ |
3555-63
|
| 公開日 |
2011-5-1
|
| DOI |
10.1016/j.biomaterials.2010.12.063
|
| PII |
S0142-9612(11)00086-X
|
| PMID |
21315447
|
| MeSH |
Animals
Blotting, Western
Cell Differentiation / drug effects
Cell Line
Cell Proliferation / drug effects
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / drug effects*
Enzyme-Linked Immunosorbent Assay
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects*
Leukemia Inhibitory Factor / chemistry
Leukemia Inhibitory Factor / pharmacology*
Mice
STAT3 Transcription Factor / metabolism
|
| IF |
10.273
|
| 引用数 |
18
|
| リソース情報 |
| 研究用ヒト臍帯血幹細胞 |
|
