論文 - 詳細
| RRC ID | 55801 |
|---|---|
| 著者 | Raghunathan N, Kapshikar RM, Leela JK, Mallikarjun J, Bouloc P, Gowrishankar J. |
| タイトル | Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Transcription termination by Rho is essential for viability in various bacteria, including some major pathogens. Since Rho acts by targeting nascent RNAs that are not simultaneously translated, it also regulates antisense transcription. Here we show that RNase H-deficient mutants of Escherichia coli exhibit heightened sensitivity to the Rho inhibitor bicyclomycin, and that Rho deficiency provokes increased formation of RNA-DNA hybrids (R-loops) which is ameliorated by expression of the phage T4-derived R-loop helicase UvsW. We also provide evidence that in Rho-deficient cells, R-loop formation blocks subsequent rounds of antisense transcription at more than 500 chromosomal loci. Hence these antisense transcripts, which can extend beyond 10 kb in their length, are only detected when Rho function is absent or compromised and the UvsW helicase is concurrently expressed. Thus the potential for antisense transcription in bacteria is much greater than hitherto recognized; and the cells are able to retain viability even when nearly one-quarter of their total non-rRNA abundance is accounted for by antisense transcripts, provided that R-loop formation from them is curtailed. |
| 巻・号 | 46(7) |
| ページ | 3400-3411 |
| 公開日 | 2018-4-20 |
| DOI | 10.1093/nar/gky118 |
| PII | 4883327 |
| PMID | 29474582 |
| PMC | PMC5909445 |
| MeSH | Bacteriophage T4 / genetics Bridged Bicyclo Compounds, Heterocyclic / pharmacology Chromosomes / genetics DNA Helicases / genetics DNA Replication / genetics DNA, Antisense / genetics Escherichia coli / drug effects Escherichia coli / genetics Genes, rRNA / genetics Genome, Bacterial / drug effects Genome, Bacterial / genetics* Rho Factor / antagonists & inhibitors Rho Factor / genetics* Ribonuclease H / genetics Transcription Termination, Genetic* Transcription, Genetic* Viral Proteins / genetics |
| IF | 11.147 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | MG1655 |