RRC ID 55814
Author Yura T, Miyazaki R, Fujiwara K, Ito K, Chiba S, Mori H, Akiyama Y.
Title Heat shock transcription factor σ32 defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in Escherichia coli.
Journal Genes Genet Syst
Abstract Heat shock transcription factor σ32 of Escherichia coli plays a major role in protein homeostasis and requires membrane localization for regulation. We here report that a strongly deregulated I54N-σ32 mutant defective in association with the membrane can be phenotypically suppressed by Tn5 insertion into the mcrC or ydbA2 gene, encoding a restriction enzyme subunit or part of a putative autotransporter, respectively. The suppression is specific for mutant I54N-σ32 and reduces its activity but not its abundance or stability. Moreover, the deregulated phenotype of I54N-σ32 is effectively suppressed by a plasmid carrying the same mcrC::Tn5 mutation. In contrast, deletion of the mcrC or ydbA2 gene hardly affects I54N-σ32 activity. These results, taken together, suggest that the truncated form of McrC (and presumably also of YdbA2) protein produced by the Tn5 insertion interacts specifically with I54N-σ32 to reduce its activity without substantially affecting its amount or stability.
Volume 93(6)
Pages 229-235
Published 2019-1-19
DOI 10.1266/ggs.18-00040
PMID 30531155
MeSH DNA Restriction Enzymes / genetics DNA Restriction Enzymes / metabolism* DNA Transposable Elements Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Heat Shock Transcription Factors / genetics* Heat Shock Transcription Factors / metabolism Recombination, Genetic Suppression, Genetic*
IF 0.859
Times Cited 1
Prokaryotes E. coli ∆mcrC, ∆ydbA2