RRC ID 55869
Author Clark T, Hapiak V, Oakes M, Mills H, Komuniecki R.
Title Monoamines differentially modulate neuropeptide release from distinct sites within a single neuron pair.
Journal PLoS One
Abstract Monoamines and neuropeptides often modulate the same behavior, but monoaminergic-peptidergic crosstalk remains poorly understood. In Caenorhabditis elegans, the adrenergic-like ligands, tyramine (TA) and octopamine (OA) require distinct subsets of neuropeptides in the two ASI sensory neurons to inhibit nociception. TA selectively increases the release of ASI neuropeptides encoded by nlp-14 or nlp-18 from either synaptic/perisynaptic regions of ASI axons or the ASI soma, respectively, and OA selectively increases the release of ASI neuropeptides encoded by nlp-9 asymmetrically, from only the synaptic/perisynaptic region of the right ASI axon. The predicted amino acid preprosequences of genes encoding either TA- or OA-dependent neuropeptides differed markedly. However, these distinct preprosequences were not sufficient to confer monoamine-specificity and additional N-terminal peptide-encoding sequence was required. Collectively, our results demonstrate that TA and OA specifically and differentially modulate the release of distinct subsets of neuropeptides from different subcellular sites within the ASIs, highlighting the complexity of monoaminergic/peptidergic modulation, even in animals with a relatively simple nervous system.
Volume 13(5)
Pages e0196954
Published 2018-5-3
DOI 10.1371/journal.pone.0196954
PII PONE-D-18-05632
PMID 29723289
PMC PMC5933757
MeSH 1-Octanol Amino Acid Sequence Animals Avoidance Learning / drug effects Avoidance Learning / physiology Behavior, Animal Caenorhabditis elegans / drug effects* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation Neuropeptides / biosynthesis Neuropeptides / genetics Neuropeptides / metabolism* Nociception / drug effects* Nociception / physiology Octopamine / pharmacology* Sensory Receptor Cells / cytology Sensory Receptor Cells / drug effects* Sensory Receptor Cells / metabolism Synapses / drug effects Synapses / physiology Tyramine / pharmacology*
IF 2.776
Times Cited 2
C.elegans tm3572 tm1880