論文 - 詳細
| RRC ID | 5589 |
|---|---|
| 著者 | Esaki M, Hoshijima K, Nakamura N, Munakata K, Tanaka M, Ookata K, Asakawa K, Kawakami K, Wang W, Weinberg ES, Hirose S. |
| タイトル | Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae. |
| ジャーナル | Dev Biol |
| Abstract |
Mitochondrion-rich cells (MRCs), or ionocytes, play a central role in aquatic species, maintaining body fluid ionic homeostasis by actively taking up or excreting ions. Since their first description in 1932 in eel gills, extensive morphological and physiological analyses have yielded important insights into ionocyte structure and function, but understanding the developmental pathway specifying these cells remains an ongoing challenge. We previously succeeded in identifying a key transcription factor, Foxi3a, in zebrafish larvae by database mining. In the present study, we analyzed a zebrafish mutant, quadro (quo), deficient in foxi1 gene expression and found that foxi1 is essential for development of an MRC subpopulation rich in vacuolar-type H(+)-ATPase (vH-MRC). foxi1 acts upstream of Delta-Notch signaling that determines sporadic distribution of vH-MRC and regulates foxi3a expression. Through gain- and loss-of-function assays and cell transplantation experiments, we further clarified that (1) the expression level of foxi3a is maintained by a positive feedback loop between foxi3a and its downstream gene gcm2 and (2) Foxi3a functions cell-autonomously in the specification of vH-MRC. These observations provide a better understanding of the differentiation and distribution of the vH-MRC subtype. |
| 巻・号 | 329(1) |
| ページ | 116-29 |
| 公開日 | 2009-5-1 |
| DOI | 10.1016/j.ydbio.2009.02.026 |
| PII | S0012-1606(09)00159-6 |
| PMID | 19268451 |
| PMC | PMC2751791 |
| MeSH | Animals Animals, Genetically Modified Fluorescent Antibody Technique, Indirect Immunohistochemistry In Situ Hybridization Keratinocytes / cytology Keratinocytes / metabolism* Larva / metabolism* Microinjections Mitochondria / metabolism* Models, Biological Oligonucleotides, Antisense / pharmacology Proton-Translocating ATPases / metabolism* Skin / cytology Skin / metabolism* Vacuoles / genetics Vacuoles / metabolism* Zebrafish / genetics Zebrafish / metabolism |
| IF | 2.896 |
| 引用数 | 51 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(UAS:GFP) |