RRC ID 5590
Author Chen YC, Cheng CH, Chen GD, Hung CC, Yang CH, Hwang SP, Kawakami K, Wu BK, Huang CJ.
Title Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene.
Journal Dev. Dyn.
Abstract Human synuclein family consists of alpha-, beta-, and gamma-synucleins. Here, we cloned three genes, sncb, sncga and sncgb from zebrafish. They encode beta-, gamma1-, and gamma2-synucleins, respectively. The zSyn-beta, zSyn-gamma1, and zSyn-gamma2 proteins display 69%, 47%, and 50% identity to human beta-synuclein and gamma-synuclein, respectively. By reverse transcriptase-polymerase chain reaction, we demonstrated that sncb and sncga mRNA were abundant in brain and eye, while sncgb expression was moderate in brain, kidney, ovary and testis. The 1.8-kb 5'-upstream/promoter region of the sncga gene was sufficient to direct green fluorescent protein (GFP) expression in the central nervous system and cranial ganglions. A transgenic line, Tg(sncga:GFP), was generated and its GFP expression is similar to that of endogenous sncga mRNA. Moreover, this line also labels the habenular complex and the domain of GFP expression is larger in the left than in the right habenula. Thus, this line can be used to study sncga gene regulation and for left-right asymmetry study in zebrafish brain.
Volume 238(3)
Pages 746-54
Published 2009-3
DOI 10.1002/dvdy.21877
PMID 19235732
MeSH Aging / physiology Amino Acid Sequence Animals Animals, Genetically Modified Cloning, Molecular Conserved Sequence Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Gene Expression Regulation, Developmental / genetics* Genes, Reporter / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Habenula / embryology Habenula / growth & development Habenula / metabolism* Humans Molecular Sequence Data Phylogeny Promoter Regions, Genetic / genetics Protein Binding Sequence Alignment Synucleins / chemistry Synucleins / genetics Synucleins / metabolism* Zebrafish / embryology Zebrafish / genetics Zebrafish / growth & development Zebrafish / metabolism* Zebrafish Proteins / chemistry Zebrafish Proteins / genetics Zebrafish Proteins / metabolism*
IF 2.507
Times Cited 15
Zebrafish ?