RRC ID 5592
Author Urasaki A, Kawakami K.
Title Analysis of genes and genome by the tol2-mediated gene and enhancer trap methods.
Journal Methods Mol. Biol.
Abstract The Tol2 transposon system can create insertions in the zebrafish genome efficiently. By using this system, the gene trap and enhancer trap methods have been developed. The gene trap and enhancer trap constructs contain the green fluorescent protein (GFP) reporter gene or the yeast Gal4 transcription activator gene. By creating random integrations of these constructs in the genome, transgenic fish expressing the GFP gene or the Gal4 gene in specific cells, tissues or organs are generated. These fish are valuable resources for developmental biology. Especially, the Gal4-expressing transgenic fish can be used to ectopically express any gene of interest placed downstream of the Gal4 recognition sequence, UAS, and thereby allow visualization, modification or ablation of the Gal4-expressing cells. In this chapter, we will describe how the gene trap and enhancer trap screens can be performed and how the transposon insertions created by these methods can be analyzed.
Volume 546
Pages 85-102
Published 2009
DOI 10.1007/978-1-60327-977-2_6
PMID 19378099
MeSH Animals Animals, Genetically Modified Crosses, Genetic DNA / genetics DNA / metabolism DNA Mutational Analysis / methods* DNA Transposable Elements* DNA-Binding Proteins Enhancer Elements, Genetic* Gene Expression Regulation, Developmental Genes, Reporter Green Fluorescent Proteins Mutagenesis, Insertional / methods* Rabbits Saccharomyces cerevisiae Proteins Transcription Factors Zebrafish / embryology Zebrafish / genetics*
Zebrafish ?