RRC ID 55932
著者 Sawai H.
タイトル Desipramine-induced lysosomal vacuolization is independent of autophagy.
ジャーナル Cell Biol Int
Abstract Desipramine, a commonly used antidepressant drug, induced cytosolic vacuolization in L929 cells. The level of LC3-II was elevated and that of p62 was reduced in desipramine-treated L929 cells, indicating the induction of autophagy by desipramine. Surprisingly, massive vacuolization was observed in desipramine-treated L929 cells in the presence of LY294002, an inhibitor of autophagy. On the other hand, bafilomycin A1, an inhibitor of vacuolar type H+ ATPase, almost completely inhibited vacuolization in desipramine- or desipramine/LY294002-treated L929 cells. Furthermore, desipramine-induced vacuolization was observed in autophagy-deficient Atg7-/- mouse embryonic fibroblasts (MEFs) as well as wild-type Atg7+/+ MEFs. These results demonstrate that desipramine-induced lysosomal vacuolization is independent of autophagy.
巻・号 42(2)
ページ 248-253
公開日 2018-2-1
DOI 10.1002/cbin.10901
PMID 29068103
MeSH Animals Antidepressive Agents, Tricyclic / pharmacology* Autophagy Autophagy-Related Protein 7 / genetics Cell Line, Tumor Cells, Cultured Desipramine / pharmacology* Lysosomes / drug effects* Macrolides / pharmacology Mice Vacuoles / drug effects*
IF 2.127
引用数 4
リソース情報
ヒト・動物細胞 Atg7 +/+ MEFs(RCB3706) Atg7 -/- MEFs(RCB3707)