RRC ID 5594
Author Kitaguchi T, Kawakami K, Kawahara A.
Title Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis.
Journal Mech. Dev.
Abstract lysozyme C (lyz), a glycoside hydrolase expressed exclusively in myeloid cells, is involved in the host defense against bacterial infection. We isolated a 2.4kb zebrafish lyz promoter region and established transgenic lines that drive enhanced green fluorescent protein (EGFP) to examine how lyz expression is regulated during myelopoiesis. We found that the 2.4kb lyz promoter is sufficient to drive myeloid-specific expression of EGFP in zebrafish. We identified potential transcriptional regulatory elements including a Runx element (TGTGGT at -1.70kb) and a C/ebp element (TTTGGCAAT at -1.46kb) in the lyz promoter, and showed that they are required for myeloid-specific expression of EGFP. We found that the myeloid-lineage transcription factors C/ebp1, Runx1 and Pu.1 can bind to the 2.4kb lyz promoter. Forced expression of runx1, c/ebp1 and pu.1 together induced ectopic lyz expression in the intermediated cell mass (ICM). Thus, we propose that c/ebp1 and runx1 presumably cooperated with pu.1 in the transcriptional regulation of lyz during zebrafish myelopoiesis.
Volume 126(5-6)
Pages 314-23
Published 2009-5
DOI 10.1016/j.mod.2009.02.007
PII S0925-4773(09)00033-1
PMID 19275935
MeSH Animals Animals, Genetically Modified Base Sequence Cell Lineage* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / enzymology Erythroid Cells / cytology Gene Expression Regulation, Developmental* Green Fluorescent Proteins / metabolism Models, Biological Molecular Sequence Data Muramidase / genetics* Muramidase / metabolism Myeloid Cells / enzymology* Myelopoiesis / genetics* Organ Specificity / genetics Promoter Regions, Genetic / genetics Transcription, Genetic* Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism
IF 1.9
Times Cited 16
Zebrafish Tg(lysC:EGFP)/ko02