RRC ID 55953
Author Tamai R, Kobayashi-Sakamoto M, Kiyoura Y.
Title Extracellular galectin-1 enhances adhesion to and invasion of oral epithelial cells by Porphyromonas gingivalis.
Journal Can J Microbiol
Abstract Galectin-1 and galectin-3 are C-type lectin receptors that bind to lipopolysaccharide in the cell wall of gram-negative bacteria. In this study, we investigated the effects of galectin-1 and galectin-3 on adhesion to and invasion of the human gingival epithelial cell line Ca9-22 by Porphyromonas gingivalis, a periodontal pathogenic gram-negative bacterium. Recombinant galectin-1, but not galectin-3, enhanced P. gingivalis adhesion and invasion, although both galectins bound similarly to P. gingivalis. Flow cytometry also revealed that Ca9-22 cells express low levels of galectin-1 and moderate levels of galectin-3. Ca9-22 cells in which galectin-3 was knocked-down did not exhibit enhanced P. gingivalis adhesion and invasion. Similarly, specific antibodies to galectin-1 and galectin-3 did not inhibit P. gingivalis adhesion and invasion. These results suggest that soluble galectin-1, but not galectin-3, may exacerbate periodontal disease by enhancing the adhesion to and invasion of host cells by periodontal pathogenic bacteria.
Volume 64(7)
Pages 465-471
Published 2018-7-1
DOI 10.1139/cjm-2017-0461
PMID 29544077
MeSH Bacterial Adhesion* / genetics Blood Proteins Cell Line Epithelial Cells / metabolism Epithelial Cells / microbiology* Galectin 1 / antagonists & inhibitors Galectin 1 / genetics Galectin 1 / metabolism* Galectin 3 / antagonists & inhibitors Galectin 3 / genetics Galectin 3 / metabolism Galectins Gene Knockdown Techniques Gingiva / cytology Gingiva / metabolism Gingiva / microbiology* Humans Porphyromonas gingivalis / metabolism Porphyromonas gingivalis / physiology* Recombinant Proteins / genetics Recombinant Proteins / metabolism
IF 1.55
Times Cited 0
Human and Animal Cells Ca9-22(RCB1976)