論文 - 詳細
| RRC ID | 55953 |
|---|---|
| 著者 | Tamai R, Kobayashi-Sakamoto M, Kiyoura Y. |
| タイトル | Extracellular galectin-1 enhances adhesion to and invasion of oral epithelial cells by Porphyromonas gingivalis. |
| ジャーナル | Can J Microbiol |
| Abstract |
Galectin-1 and galectin-3 are C-type lectin receptors that bind to lipopolysaccharide in the cell wall of gram-negative bacteria. In this study, we investigated the effects of galectin-1 and galectin-3 on adhesion to and invasion of the human gingival epithelial cell line Ca9-22 by Porphyromonas gingivalis, a periodontal pathogenic gram-negative bacterium. Recombinant galectin-1, but not galectin-3, enhanced P. gingivalis adhesion and invasion, although both galectins bound similarly to P. gingivalis. Flow cytometry also revealed that Ca9-22 cells express low levels of galectin-1 and moderate levels of galectin-3. Ca9-22 cells in which galectin-3 was knocked-down did not exhibit enhanced P. gingivalis adhesion and invasion. Similarly, specific antibodies to galectin-1 and galectin-3 did not inhibit P. gingivalis adhesion and invasion. These results suggest that soluble galectin-1, but not galectin-3, may exacerbate periodontal disease by enhancing the adhesion to and invasion of host cells by periodontal pathogenic bacteria. |
| 巻・号 | 64(7) |
| ページ | 465-471 |
| 公開日 | 2018-7-1 |
| DOI | 10.1139/cjm-2017-0461 |
| PMID | 29544077 |
| MeSH | Bacterial Adhesion* / genetics Blood Proteins Cell Line Epithelial Cells / metabolism Epithelial Cells / microbiology* Galectin 1 / antagonists & inhibitors Galectin 1 / genetics Galectin 1 / metabolism* Galectin 3 / antagonists & inhibitors Galectin 3 / genetics Galectin 3 / metabolism Galectins Gene Knockdown Techniques Gingiva / cytology Gingiva / metabolism Gingiva / microbiology* Humans Porphyromonas gingivalis / metabolism Porphyromonas gingivalis / physiology* Recombinant Proteins / genetics Recombinant Proteins / metabolism |
| IF | 1.55 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) |