RRC ID 5597
Author Lillesaar C, Tannhäuser B, Stigloher C, Kremmer E, Bally-Cuif L.
Title The serotonergic phenotype is acquired by converging genetic mechanisms within the zebrafish central nervous system.
Journal Dev. Dyn.
Abstract To gain knowledge about the developmental origin of serotonergic precursors and the regulatory cascades of serotonergic differentiation in vertebrates, we determined the spatiotemporal expression profile of the Ets-domain transcription factor-encoding gene pet1 in developing and adult zebrafish. We show that it is an early, specific marker of raphe serotonergic neurons, but not of other serotonergic populations. We then use pet1 expression together with tracing techniques to demonstrate that serotonergic neurons of rhombomeres (r) 1-2 largely originate from a progenitor pool at the midbrain-hindbrain boundary. Furthermore, by combining expression analyses of pet1 and the raphe tryptophan hydroxylase (Tph2) with rhombomere identity markers, we show that anterior and posterior hindbrain clusters of serotonergic precursors are separated by r3, rather than r4 as in other vertebrates. Our findings establish the origin of r1-2 serotonergic precursors, and strengthen the evidence for molecular, ontogenic and phylogenic heterogeneities among the vertebrate brain serotonergic cell populations.
Volume 236(4)
Pages 1072-84
Published 2007-4
DOI 10.1002/dvdy.21095
PMID 17304529
MeSH Amino Acid Sequence Animals Base Sequence Cell Differentiation Central Nervous System / embryology* Central Nervous System / growth & development Embryo, Nonmammalian Gene Expression Regulation, Developmental Models, Biological Molecular Sequence Data Neurons / metabolism* Neurons / physiology* Phenotype* Phylogeny Sequence Homology, Amino Acid Serotonin / metabolism* Transcription Factors / genetics Zebrafish / embryology* Zebrafish / genetics Zebrafish / growth & development Zebrafish Proteins / genetics
IF 2.852
Times Cited 43
WOS Category ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY
Resource
Zebrafish rw0 (Tg(CM-isl1:GFP))?