RRC ID 5602
Author Clarke JD, Jayasinghe SN.
Title Bio-electrosprayed multicellular zebrafish embryos are viable and develop normally.
Journal Biomed Mater
Abstract Bio-electrosprays are rapidly emerging as a viable protocol for directly engineering living cells. This communication reports the bio-electrospraying of multicellular organisms, namely zebrafish embryos. The results demonstrate that the bio-electrospray protocol fails to induce any embryological perturbations. In addition to analysing overall embryo morphology, we use transgenic embryos that express green fluorescent protein in specific brain neurons to determine that neuronal numbers and organization are completely normal. These results demonstrate that the bio-electrospraying protocol does not interfere with the complex gene regulation and cell movements required for the development of a multicellular organism.
Volume 3(1)
Pages 011001
Published 2008-3-1
DOI 10.1088/1748-6041/3/1/011001
PII S1748-6041(08)64090-5
PMID 18458487
MeSH Animals Cell Survival Electrochemistry / methods* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / physiology* Embryonic Development / physiology* Fetal Viability / physiology Microfluidics / methods* Organ Culture Techniques / methods* Pressure Tissue Engineering / methods Zebrafish / anatomy & histology Zebrafish / embryology* Zebrafish / physiology*
IF 3.174
Times Cited 29
Zebrafish rw0 (Tg(Islet-1:GFP))?