| RRC ID |
56044
|
| 著者 |
Aota K, Yamanoi T, Kani K, Azuma M.
|
| タイトル |
Cepharanthine Inhibits IFN-γ-Induced CXCL10 by Suppressing the JAK2/STAT1 Signal Pathway in Human Salivary Gland Ductal Cells.
|
| ジャーナル |
Inflammation
|
| Abstract |
Cepharanthine, a biscolaurine alkaloid isolated from the plant Stephania cephalantha Hayata, has been reported to have potent anti-inflammatory properties. Here, we investigated the effects of cepharanthine on the expression of CXCL10 (a CXC chemokine induced by interferon-gamma [IFN-γ] that has been observed in a wide variety of chronic inflammatory disorders and autoimmune conditions) in IFN-γ-treated human salivary gland cell lines. We observed that IFN-γ-induced CXCL10 production in NS-SV-DC cells (a human salivary gland ductal cell line), but not in NS-SV-AC cells (a human salivary gland acinar cell line). Cepharanthine inhibited the IFN-γ-induced CXCL10 production in NS-SV-DC cells. A Western blot analysis showed that cepharanthine prevented the phosphorylation of JAK2 and STAT1, but did not interfere with the NF-κB pathway. Moreover, cepharanthine inhibited the IFN-γ-mediated chemotaxis of Jurkat T cells. These results suggest that cepharanthine suppresses IFN-γ-induced CXCL10 production via the inhibition of the JAK2/STAT1 signaling pathway in human salivary gland ductal cells. Our findings also indicate that cepharanthine could inhibit the chemotaxis of Jurkat T cells by reducing CXCL10 production.
|
| 巻・号 |
41(1)
|
| ページ |
50-58
|
| 公開日 |
2018-2-1
|
| DOI |
10.1007/s10753-017-0662-x
|
| PII |
10.1007/s10753-017-0662-x
|
| PMID |
28879548
|
| MeSH |
Anti-Inflammatory Agents / pharmacology*
Benzylisoquinolines / pharmacology*
Chemokine CXCL10 / metabolism*
Chemotaxis, Leukocyte / drug effects
Dose-Response Relationship, Drug
Humans
Interferon-gamma / pharmacology*
Janus Kinase 2 / metabolism*
Jurkat Cells
NF-kappa B / metabolism
Phosphorylation
STAT1 Transcription Factor / metabolism*
Salivary Ducts / drug effects*
Salivary Ducts / metabolism
Signal Transduction / drug effects
Time Factors
|
| IF |
2.939
|
| 引用数 |
5
|
| リソース情報 |
| ヒト・動物細胞 |
Jurkat |
