RRC ID 5608
Author Sittaramane V, Sawant A, Wolman MA, Maves L, Halloran MC, Chandrasekhar A.
Title The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish.
Journal Dev. Biol.
Abstract Interactions between a neuron and its environment play a major role in neuronal migration. We show here that the cell adhesion molecule Transient Axonal Glycoprotein (Tag1) is necessary for the migration of the facial branchiomotor neurons (FBMNs) in the zebrafish hindbrain. In tag1 morphant embryos, FBMN migration is specifically blocked, with no effect on organization or patterning of other hindbrain neurons. Furthermore, using suboptimal morpholino doses and genetic mutants, we found that tag1, lamininalpha1 (lama1) and stbm, which encodes a transmembrane protein Vangl2, exhibit pairwise genetic interactions for FBMN migration. Using time-lapse analyses, we found that FBMNs are affected similarly in all three single morphant embryos, with an inability to extend protrusions in a specific direction, and resulting in the failure of caudal migration. These data suggest that tag1, lama1 and vangl2 participate in a common mechanism that integrates signaling between the FBMN and its environment to regulate migration.
Volume 325(2)
Pages 363-73
Published 2009-1-15
DOI 10.1016/j.ydbio.2008.10.030
PII S0012-1606(08)01294-3
PMID 19013446
PMC PMC2991145
MeSH Animals Cell Adhesion Cell Adhesion Molecules, Neuronal / physiology* Cell Movement Contactin 2 Laminin / physiology* Membrane Proteins / physiology* Motor Neurons / physiology* Rhombencephalon / embryology* Rhombencephalon / physiology Signal Transduction Zebrafish / embryology* Zebrafish / physiology Zebrafish Proteins / physiology*
IF 3.262
Times Cited 32
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Zebrafish rw0(Tg(isl1:GFP))