RRC ID 56082
Author Tsai WH, Yu KH, Huang YC, Lee CI.
Title EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles.
Journal Int J Nanomedicine
Abstract Background:Photodynamic therapy (PDT) is an effective therapy for cancers and is a minimally invasive therapy with low dark toxicity and limited side effects. PDT employs the combination of photosensitizers with a specific light source to produce reactive oxygen species (ROS) to damage tumor cells.
Methods:We fabricated nanoparticles encapsulating curcumin through crosslinking chitosan and tripolyphosphate (TPP). Additionally, the chitosan was conjugated to epidermal growth factor in order to target the epidermal growth factor receptor (EGFR), overexpressed on cancer cells. To investigate PDT using fabricated nanoparticles, we measured cell viabilities and ROS production in relation to EGFR-overexpressing gastric cancer cells and non-cancer gastric cells.
Results:The targeting nanoparticles displayed a superior PDT effect in the cancer cell, with a resultant approximately fourfold decrease in the IC50. The PDT mechanism of curcumin-encapsulated nanoparticles is further identified as the generation of 1O2, the major pathway in PDT.
Conclusion:These curcumin-encapsulated chitosan/TPP nanoparticles are a promising targeted-PDT against EGFR-overexpressing cancers.
Volume 13
Pages 903-916
Published 2018
DOI 10.2147/IJN.S148305
PII ijn-13-903
PMID 29445279
PMC PMC5810525
MeSH Apoptosis / drug effects Cell Line, Tumor Cell Survival / drug effects Chitosan / chemistry* Curcumin / chemistry Curcumin / pharmacology* Epidermal Growth Factor / pharmacology ErbB Receptors / metabolism* Flow Cytometry Humans Interleukin-10 / metabolism Nanoparticles / chemistry* Necrosis Photochemotherapy* Photosensitizing Agents / pharmacology Polyphosphates / chemistry* Reactive Oxygen Species / metabolism Spectroscopy, Fourier Transform Infrared Superoxides / metabolism
Resource
Human and Animal Cells MKN45(RCB1001)