| RRC ID |
56084
|
| 著者 |
Afroz S, Yagi A, Fujikawa K, Rahman MM, Morito K, Fukuta T, Watanabe S, Kiyokage E, Toida K, Shimizu T, Ishida T, Kogure K, Tokumura A, Tanaka T.
|
| タイトル |
Lysophosphatidic acid in medicinal herbs enhances prostaglandin E2 and protects against indomethacin-induced gastric cell damage in vivo and in vitro.
|
| ジャーナル |
Prostaglandins Other Lipid Mediat
|
| Abstract |
Lysophosphatidic acid (LPA) is a bioactive phospholipid that induces diverse biological responses. Recently, we found that LPA ameliorates NSAIDs-induced gastric ulcer in mice. Here, we quantified LPA in 21 medicinal herbs used for treatment of gastrointestinal (GI) disorders. We found that half of them contained LPA at relatively high levels (40-240 μg/g) compared to soybean seed powder (4.6 μg/g), which we previously identified as an LPA-rich food. The LPA in peony (Paeonia lactiflora) root powder is highly concentrated in the lipid fraction that ameliorates indomethacin-induced gastric ulcer in mice. Synthetic 18:1 LPA, peony root LPA and peony root lipid enhanced prostaglandin E2 production in a gastric cancer cell line, MKN74 cells that express LPA2 abundantly. These materials also prevented indomethacin-induced cell death and stimulated the proliferation of MKN74 cells. We found that LPA was present in stomach fluids at 2.4 μM, which is an effective LPA concentration for inducing a cellular response in vitro. These results indicated that LPA is one of the active components of medicinal herbs for the treatment of GI disorder and that orally administered LPA-rich herbs may augment the protective actions of endogenous LPA on gastric mucosa.
|
| 巻・号 |
135
|
| ページ |
36-44
|
| 公開日 |
2018-3-1
|
| DOI |
10.1016/j.prostaglandins.2018.01.003
|
| PII |
S1098-8823(17)30140-5
|
| PMID |
29462674
|
| MeSH |
Animals
Cell Death / drug effects
Cell Proliferation / drug effects
Dinoprostone / metabolism*
Indomethacin / adverse effects*
Lysophospholipids / therapeutic use*
Mice
Plants, Medicinal / chemistry*
Signal Transduction / drug effects
Stomach Ulcer / chemically induced
Stomach Ulcer / drug therapy
Stomach Ulcer / metabolism
|
| IF |
2.253
|
| 引用数 |
9
|
| リソース情報 |
| ヒト・動物細胞 |
MKN74(RCB1002) |
