| RRC ID |
56099
|
| Author |
Matsushima Y, Terada K, Takata J, Karube Y, Kamei C, Sugimoto Y.
|
| Title |
Effects of fluvoxamine on nerve growth factor-induced neurite outgrowth inhibition by dexamethasone in PC12 cells.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
In the present study, we examined the effects of fluvoxamine on nerve growth factor (NGF)-induced neurite outgrowth inhibition by dexamethasone (DEX) in PC12 cells. Fluvoxamine increased NGF-induced neurite outgrowth. Compared with co-treatment with NGF and fluvoxamine, p-Akt levels were higher than the values without fluvoxamine. The phosphorylated extracellular regulated kinase 1/2 levels were slightly increased by co-treatment with NGF and fluvoxamine. Fluvoxamine concentration-dependently improved NGF-induced neurite outgrowth inhibition by DEX. Fluvoxamine also improved the decrease in the NGF-induced p-Akt level caused by DEX. Interestingly, the sigma-1 receptor antagonist NE-100 blocked the improvement effects of fluvoxamine on NGF-induced neurite outgrowth inhibition by DEX. The selective sigma-1 receptor agonist PRE-084 also improved NGF-induced neurite outgrowth inhibition by DEX, which is blocked by NE-100. These results indicate that the improvement effects of fluvoxamine on NGF-induced neurite outgrowth inhibition by DEX may be attributable to the phosphorylation of Akt and the sigma-1 receptor.
|
| Volume |
83(4)
|
| Pages |
659-665
|
| Published |
2019-4-1
|
| DOI |
10.1080/09168451.2018.1553607
|
| PMID |
30543144
|
| MeSH |
Animals
Anisoles / pharmacology
Anti-Anxiety Agents / pharmacology*
Cell Differentiation / drug effects
Dexamethasone / antagonists & inhibitors
Dexamethasone / pharmacology
Fluvoxamine / pharmacology*
Gene Expression Regulation / drug effects*
Glucocorticoids / antagonists & inhibitors
Glucocorticoids / pharmacology
Mitogen-Activated Protein Kinase 1 / genetics
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / genetics
Mitogen-Activated Protein Kinase 3 / metabolism
Morpholines / pharmacology
Nerve Growth Factor / pharmacology
Neuronal Outgrowth / drug effects*
Neuronal Outgrowth / genetics
Neurons / cytology
Neurons / drug effects*
Neurons / metabolism
PC12 Cells
Phosphorylation / drug effects
Propylamines / pharmacology
Proto-Oncogene Proteins c-akt / agonists
Proto-Oncogene Proteins c-akt / antagonists & inhibitors
Proto-Oncogene Proteins c-akt / genetics*
Proto-Oncogene Proteins c-akt / metabolism
Rats
Receptors, sigma / agonists
Receptors, sigma / antagonists & inhibitors
Receptors, sigma / genetics
Receptors, sigma / metabolism
Signal Transduction
|
| IF |
1.297
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
