| RRC ID |
56151
|
| 著者 |
Nakatake R, Kaibori M, Nakamura Y, Tanaka Y, Matushima H, Okumura T, Murakami T, Ino Y, Todo T, Kon M.
|
| タイトル |
Third-generation oncolytic herpes simplex virus inhibits the growth of liver tumors in mice.
|
| ジャーナル |
Cancer Sci
|
| Abstract |
Multimodality therapies are used to manage patients with hepatocellular carcinoma (HCC), although advanced HCC is incurable. Oncolytic virus therapy is probably the next major breakthrough in cancer treatment. The third-generation oncolytic herpes simplex virus type 1 (HSV-1) T-01 kills tumor cells without damaging the surrounding normal tissues. Here we investigated the antitumor effects of T-01 on HCC and the host's immune response to HCC cells. The cytopathic activities of T-01 were tested in 14 human and 1 murine hepatoma cell line in vitro. In various mouse xenograft models, HuH-7, KYN-2, PLC/PRF/5 and HepG2 human cells and Hepa1-6 murine cells were used to investigate the in vivo efficacy of T-01. T-01 was cytotoxic to 13 cell lines (in vitro). In mouse xenograft models of subcutaneous, orthotopic and peritoneal tumor metastasis in athymic mice (BALB/c nu/nu), the growth of tumors formed by the human HCC cell lines and hepatoblastoma cell line was inhibited by T-01 compared with that of mock-inoculated tumors. In a bilateral Hepa1-6 subcutaneous tumor model in C57BL/6 mice, the growth of tumors inoculated with T-01 was inhibited, as was the case for contralateral tumors. T-01 also significantly reduced tumor growth. T-01 infection significantly enhanced antitumor efficacy via T cell-mediated immune responses. Results demonstrate that a third-generation oncolytic HSV-1 may serve as a novel treatment for patients with HCC.
|
| 巻・号 |
109(3)
|
| ページ |
600-610
|
| 公開日 |
2018-3-1
|
| DOI |
10.1111/cas.13492
|
| PMID |
29288515
|
| PMC |
PMC5834814
|
| MeSH |
Animals
Carcinoma, Hepatocellular / immunology
Carcinoma, Hepatocellular / therapy*
Hep G2 Cells
Herpesvirus 1, Human / physiology*
Humans
Liver Neoplasms / immunology
Liver Neoplasms / therapy*
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Oncolytic Viruses / physiology*
Peritoneal Neoplasms / immunology
Peritoneal Neoplasms / secondary
Peritoneal Neoplasms / therapy*
Treatment Outcome
Virus Replication
Xenograft Model Antitumor Assays
|
| IF |
4.751
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
Vero(RCB0001) |
