| RRC ID |
5616
|
| 著者 |
von Hofsten J, Elworthy S, Gilchrist MJ, Smith JC, Wardle FC, Ingham PW.
|
| タイトル |
Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo.
|
| ジャーナル |
EMBO Rep
|
| Abstract |
The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show that Prdm1 functions by acting as a transcriptional repressor and that slow-twitch-specific muscle gene expression is activated by Prdm1-mediated repression of the transcriptional repressor Sox6. Genes encoding fast-specific isoforms of sarcomeric proteins are ectopically expressed in the adaxial cells of ubo(tp39) mutant embryos. By using chromatin immunoprecipitation, we show that these are direct targets of Prdm1. Thus, Prdm1 promotes slow-twitch fibre differentiation by acting as a global repressor of fast-fibre-specific genes, as well as by abrogating the repression of slow-fibre-specific genes.
|
| 巻・号 |
9(7)
|
| ページ |
683-9
|
| 公開日 |
2008-7-1
|
| DOI |
10.1038/embor.2008.73
|
| PII |
embor200873
|
| PMID |
18535625
|
| PMC |
PMC2424280
|
| MeSH |
Animals
Binding Sites
Cardiac Myosins / genetics
Cell Differentiation
Chromatin Immunoprecipitation
DNA-Binding Proteins / metabolism*
Embryo, Nonmammalian / metabolism*
Gene Expression Regulation, Developmental
Muscle Fibers, Fast-Twitch / cytology
Muscle Fibers, Skeletal / cytology
Muscle Fibers, Skeletal / metabolism*
Muscle Fibers, Slow-Twitch / cytology
Mutation / genetics
Myosin Light Chains / genetics
Nuclear Proteins / metabolism*
Organ Specificity
Positive Regulatory Domain I-Binding Factor 1
Promoter Regions, Genetic / genetics
Repressor Proteins / metabolism*
Transcription, Genetic*
Up-Regulation / genetics
Zebrafish / embryology*
Zebrafish / genetics
Zebrafish Proteins / metabolism*
|
| IF |
7.497
|
| 引用数 |
92
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(alpha-actin:GFP)? |
