RRC ID 56174
Author Ishidate T, Ozturk AR, Durning DJ, Sharma R, Shen EZ, Chen H, Seth M, Shirayama M, Mello CC.
Title ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals.
Journal Mol Cell
Abstract Animal cells have a remarkable capacity to adopt durable and heritable gene expression programs or epigenetic states that define the physical properties and diversity of somatic cell types. The maintenance of epigenetic programs depends on poorly understood pathways that prevent gain or loss of inherited signals. In the germline, epigenetic factors are enriched in liquid-like perinuclear condensates called nuage. Here, we identify the deeply conserved helicase-domain protein, ZNFX-1, as an epigenetic regulator and component of nuage that interacts with Argonaute systems to balance epigenetic inheritance. Our findings suggest that ZNFX-1 promotes the 3' recruitment of machinery that propagates the small RNA epigenetic signal and thus counteracts a tendency for Argonaute targeting to shift 5' along the mRNA. These functional insights support the idea that recently identified subdomains of nuage, including ZNFX-1 granules or "Z-granules," may define spatial and temporal zones of molecular activity during epigenetic regulation.
Volume 70(4)
Pages 639-649.e6
Published 2018-5-17
DOI 10.1016/j.molcel.2018.04.009
PII S1097-2765(18)30279-X
PMID 29775580
PMC PMC5994929
MeSH Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism* Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / genetics* Epigenesis, Genetic* Germ Cells / metabolism* Organelles RNA Helicases / genetics RNA Helicases / metabolism* RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism RNA-Dependent RNA Polymerase / genetics RNA-Dependent RNA Polymerase / metabolism
IF 14.548
Times Cited 17
C.elegans tm872 tm892