Reference - Detail
| RRC ID | 56190 |
|---|---|
| Author | Le Pen J, Jiang H, Di Domenico T, Kneuss E, Kosałka J, Leung C, Morgan M, Much C, Rudolph KLM, Enright AJ, O'Carroll D, Wang D, Miska EA. |
| Title | Terminal uridylyltransferases target RNA viruses as part of the innate immune system. |
| Journal | Nat Struct Mol Biol |
| Abstract |
RNA viruses are a major threat to animals and plants. RNA interference (RNAi) and the interferon response provide innate antiviral defense against RNA viruses. Here, we performed a large-scale screen using Caenorhabditis elegans and its natural pathogen the Orsay virus (OrV), and we identified cde-1 as important for antiviral defense. CDE-1 is a homolog of the mammalian TUT4 and TUT7 terminal uridylyltransferases (collectively called TUT4(7)); its catalytic activity is required for its antiviral function. CDE-1 uridylates the 3' end of the OrV RNA genome and promotes its degradation in a manner independent of the RNAi pathway. Likewise, TUT4(7) enzymes uridylate influenza A virus (IAV) mRNAs in mammalian cells. Deletion of TUT4(7) leads to increased IAV mRNA and protein levels. Collectively, these data implicate 3'-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism. |
| Volume | 25(9) |
| Pages | 778-786 |
| Published | 2018-9-1 |
| DOI | 10.1038/s41594-018-0106-9 |
| PII | 10.1038/s41594-018-0106-9 |
| PMID | 30104661 |
| PMC | PMC6130846 |
| MeSH | A549 Cells Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans / virology* Humans Immunity, Innate* RNA Interference RNA Nucleotidyltransferases / metabolism* RNA Viruses / immunology RNA Viruses / metabolism* RNA Viruses / physiology RNA, Viral / metabolism Transcriptome Virus Replication |
| IF | 12.109 |
| Times Cited | 20 |
| Resource | |
| C.elegans | tm1021 tm1098 |