論文 - 詳細
| RRC ID | 56219 |
|---|---|
| 著者 | Scheidel N, Kennedy J, Blacque OE. |
| タイトル | Endosome maturation factors Rabenosyn-5/VPS45 and caveolin-1 regulate ciliary membrane and polycystin-2 homeostasis. |
| ジャーナル | EMBO J |
| Abstract |
Primary cilium structure and function relies on control of ciliary membrane homeostasis, regulated by membrane trafficking processes that deliver and retrieve ciliary components at the periciliary membrane. However, the molecular mechanisms controlling ciliary membrane establishment and maintenance, especially in relation to endocytosis, remain poorly understood. Here, using Caenorhabditis elegans, we describe closely linked functions for early endosome (EE) maturation factors RABS-5 (Rabenosyn-5) and VPS-45 (VPS45) in regulating cilium length and morphology, ciliary and periciliary membrane volume, and ciliary signalling-related sensory behaviour. We demonstrate that RABS-5 and VPS-45 control periciliary vesicle number and levels of select EE/endocytic markers (WDFY-2, CAV-1) and the ciliopathy membrane receptor PKD-2 (polycystin-2). Moreover, we show that CAV-1 (caveolin-1) also controls PKD-2 ciliary levels and associated sensory behaviour. These data link RABS-5 and VPS-45 ciliary functions to the processing of periciliary-derived endocytic vesicles and regulation of ciliary membrane homeostasis. Our findings also provide insight into the regulation of PKD-2 ciliary levels via integrated endosomal sorting and CAV-1-mediated endocytosis. |
| 巻・号 | 37(9) |
| 公開日 | 2018-5-2 |
| DOI | 10.15252/embj.201798248 |
| PII | embj.201798248 |
| PMID | 29572244 |
| PMC | PMC5920237 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caveolin 1 / genetics Caveolin 1 / metabolism* Cell Membrane / genetics Cell Membrane / metabolism* Cilia / genetics Cilia / metabolism TRPP Cation Channels / genetics TRPP Cation Channels / metabolism* Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 11.227 |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1215 tm0246 |