RRC ID 56228
著者 Thapliyal S, Ravindranath S, Babu K.
タイトル Regulation of Glutamate Signaling in the Sensorimotor Circuit by CASY-1A/Calsyntenin in Caenorhabditis elegans.
ジャーナル Genetics
Abstract Locomotion is one of the most prominent behaviors in the nematode Caenorhabditis elegans Neuronal circuits that ultimately produce coordinated dorso-ventral sinusoidal bends mediate this behavior. Synchronized locomotion requires an intricate balance between excitation and inhibition at the neuromuscular junctions (NMJ), the complex cellular and molecular mechanisms of which are not fully understood. Here, we describe the role of a cell adhesion molecule CASY-1, which functions to maintain this balance at the NMJ. In this study, we dissect out mechanisms by which the longer CASY-1A isoform could be affecting the excitatory cholinergic signaling at the NMJ by modulating the activity of sensory neurons. Mutants in casy-1 appear to have hyperactive sensory neurons, resulting in accelerated locomotion and motor circuit activity. These sensory neurons mediate increased motor activity via enhanced glutamate release. Using genetic, pharmacological, and optogenetic manipulations, we establish that CASY-1A is required to monitor the activity of these neurons. Our study illustrates a novel neuromodulatory role of CASY-1-mediated signaling in regulating the excitation-inhibition balance of the motor circuit.
巻・号 208(4)
ページ 1553-1564
公開日 2018-4-1
DOI 10.1534/genetics.118.300834
PII genetics.118.300834
PMID 29475851
PMC PMC5887148
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Calcium-Binding Proteins / metabolism* Feedback, Sensory* Gene Expression Regulation Glutamates / metabolism* Interneurons / metabolism Locomotion Membrane Proteins Models, Biological Molecular Imaging Motor Neurons / metabolism Mutation Neuromuscular Junction / metabolism Sensory Receptor Cells / metabolism Serine Endopeptidases Signal Transduction*
IF 3.564
引用数 2
リソース情報
線虫 tm718